Boulton M, Flessner M, Armstrong D, Hay J, Johnston M
Trauma Research Program, Sunnybrook Health Science Centre, University of Toronto, Ontario, Canada.
Am J Physiol. 1997 May;272(5 Pt 2):R1613-9. doi: 10.1152/ajpregu.1997.272.5.R1613.
The plasma recovery of an intraventricularly administered protein was compared before and after lymph diversion/ligation in the same conscious sheep to determine the relative roles of arachnoid villi and lymphatics in the clearance of a cerebral spinal fluid tracer. 125I-human serum albumin was injected into both lateral ventricles, and venous blood was sampled. One day later, multiple cervical vessels and the thoracic duct were cannulated for lymph collection. Uncannulated vessels were ligated. The experiment was repeated with 131I-human serum albumin as the tracer. Before lymph diversion/ligation, the time-averaged tracer transport into the plasma was 6.4 +/- 1.0%/h, with an average 6-h plasma recovery of 38.2 +/- 5.7% (percentage of injected dose). After lymph diversion/ligation, the values dropped to 2.9 +/- 0.5%/h and 17.7 +/- 2.7%, respectively. The collected lymph contained 8.7 +/- 2.6% of the tracer. No significant differences were observed in sham-operated animals. In conclusion, extracranial lymphatic vessels in sheep transport approximately one-half of the protein tracer from the cerebral spinal fluid compartment into plasma.
在同一清醒绵羊体内，比较了脑室注射蛋白质后蛛网膜绒毛和淋巴管在清除脑脊液示踪剂中的相对作用，该实验在淋巴分流/结扎前后进行。向双侧脑室注射¹²⁵I-人血清白蛋白，并采集静脉血样。一天后，将多条颈血管和胸导管插管用于收集淋巴液。未插管的血管进行结扎。以¹³¹I-人血清白蛋白作为示踪剂重复该实验。在淋巴分流/结扎前，示踪剂进入血浆的时间平均转运率为6.4±1.0%/小时，6小时的血浆平均回收率为38.2±5.7%（注射剂量的百分比）。淋巴分流/结扎后，这些值分别降至2.9±0.5%/小时和17.7±2.7%。收集的淋巴液中含有8.7±2.6%的示踪剂。在假手术动物中未观察到显著差异。总之，绵羊颅外淋巴管将大约一半的蛋白质示踪剂从脑脊液腔转运到血浆中。
