多巴胺会导致罗非鱼（尼罗罗非鱼）催乳素细胞的超微结构发生变化。
Dopamine causes ultrastructural changes in prolactin cells of tilapia (Oreochromis niloticus).
作者信息
Hazineh A, Shin S H, Reifel C, Pang S C, Van der Kraak G J
机构信息
Department of Physiology, Queen's University, Kingston, Ontario, Canada.
出版信息
Cell Mol Life Sci. 1997 May;53(5):452-8. doi: 10.1007/s000180050055.
This study was undertaken to examine ultrastructural changes induced by dopamine in fish prolactin cells. Tilapia adenohypophyses were incubated with dopamine and evaluated by electron microscopy. The quantities of rough endoplasmic reticulum (RER) in prolactin cells increased and the number of secretory granules were decreased by dopamine (10(-6) mol/l) treatment. Another set of adenohypophysial tissues was placed back into control medium for 10 min following a 3 h incubation period with dopamine (10(-6) mol/l) (RE10 min group). This group had significantly less RER than the 3 h dopamine-treated tissue, and the shape of many granules in the RE10 min group changed from spherical to rod-like. In addition, some of the granule content appeared to diffuse out of granules since some were not fully surrounded by membrane. It was therefore hypothesized that the rod-shaped granules might be the result of prolactin secretion by diffusion.
本研究旨在检测多巴胺对鱼类催乳素细胞诱导的超微结构变化。将罗非鱼腺垂体与多巴胺一起孵育，并通过电子显微镜进行评估。多巴胺（10⁻⁶ mol/l）处理使催乳素细胞中粗面内质网（RER）的数量增加，分泌颗粒的数量减少。另一组腺垂体组织在与多巴胺（10⁻⁶ mol/l）孵育3小时后，再放回对照培养基中10分钟（RE10分钟组）。该组的RER明显少于多巴胺处理3小时的组织，并且RE10分钟组中许多颗粒的形状从球形变为棒状。此外，一些颗粒内容物似乎从颗粒中扩散出来，因为有些颗粒没有被膜完全包围。因此，推测棒状颗粒可能是催乳素通过扩散分泌的结果。
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