Kudo Y, Shibata S, Miyaki T, Aono T, Oyaizu H
Graduate School of Agriculture and Agricultural Life Sciences, University of Tokyo, Japan.
Biosci Biotechnol Biochem. 1997 May;61(5):917-20.
Archaeal 16S rDNA clones retrieved from paddy soil DNA were sequenced. Among 100 clones, 88 clones were assigned to methanogens and nine clones were assigned to crenarchaeota. However, three of the nine clones were phylogenetically far from the cultured crenarchaeota and closely related to marine planktonic archaea. The other three clones showed extremely novel 16S rDNA sequences and were phylogenetically far from both Crenarchaeola and Euryarchaeota. This paper reports the ubiquitous presence of crenarchaeotal and extremely novel clones in paddy soils.
对从稻田土壤DNA中提取的古菌16S rDNA克隆进行了测序。在100个克隆中，88个克隆被归类为产甲烷菌，9个克隆被归类为泉古菌。然而，这9个克隆中的3个在系统发育上与已培养的泉古菌相差甚远，而与海洋浮游古菌密切相关。另外3个克隆显示出极其新颖的16S rDNA序列，在系统发育上与泉古菌门和广古菌门都相差甚远。本文报道了稻田土壤中普遍存在泉古菌和极其新颖的克隆。
