Akagi M, Fukuishi N, Kan T, Sagesaka Y M, Akagi R
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho, Japan.
Biol Pharm Bull. 1997 May;20(5):565-7. doi: 10.1248/bpb.20.565.
We investigated the anti-allergic effect of tea-leaf saponin (TLS), which was a mixture of saponins separated from the leaves of Camellia sinensis var. sinensis, in guinea pigs and rats. TLS (20-100 mg/kg) dose-dependently inhibited experimentally-induced asthma, and ID50 was 61.7 mg/kg. TLS (20-100 mg/kg) dose-dependently inhibited a 48 h homologous PCA (passive cutaneous anaphylaxis) reaction, and the inhibitory effect was similar to that of tranilast. TLS (1-100 microg/ml) also inhibited the release of antigen-induced leukotriene (LT) C4 from sensitized guinea pig lung samples in a dose-dependent fashion, but did not prevent histamine release. TLS (0.01-0.5 microg/ml) inhibited histamine release from rat peritoneal mast cells induced by compound 48/80. At higher concentrations, TLS elicited histamine release. These findings suggest that TLS may be a useful protective agent against clinical allergy, and that the inhibitory effects of TLS on mediator release are in some way related to its inhibitory effect on experimentally-induced asthma and PCA reaction.
我们研究了茶叶皂苷（TLS）对豚鼠和大鼠的抗过敏作用，TLS是从中华变种茶树叶片中分离得到的皂苷混合物。TLS（20 - 100毫克/千克）剂量依赖性地抑制实验性诱发的哮喘，半数抑制剂量（ID50）为61.7毫克/千克。TLS（20 - 100毫克/千克）剂量依赖性地抑制48小时同源被动皮肤过敏反应（PCA），其抑制作用与曲尼司特相似。TLS（1 - 100微克/毫升）还以剂量依赖性方式抑制致敏豚鼠肺样本中抗原诱导的白三烯（LT）C4释放，但不阻止组胺释放。TLS（0.01 - 0.5微克/毫升）抑制化合物48/80诱导的大鼠腹腔肥大细胞组胺释放。在较高浓度下，TLS会引发组胺释放。这些发现表明，TLS可能是一种有用的临床过敏保护剂，并且TLS对介质释放的抑制作用在某种程度上与其对实验性诱发的哮喘和PCA反应的抑制作用相关。
