茶叶籽饼（Camellia oleifera Abel）中三萜皂苷的定性和定量分析及其对细菌和真菌的活性。

Qualitative and quantitative analysis of triterpene saponins from tea seed pomace (Camellia oleifera Abel) and their activities against bacteria and fungi.

机构信息

College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China.

Key Laboratory of Tea Biochemistry & Biotechnology, Ministry of Education and Ministry of Agriculture, Anhui Agricultural University, Hefei 230036, China.

出版信息

Molecules. 2014 Jun 6;19(6):7568-80. doi: 10.3390/molecules19067568.

A method using LC-ESI-IT-TOF/MS and LC/UV-ELSD was established to qualitatively analyze triterpene saponins obtained from the tea seed pomace (Camellia oleifera Abel). In addition, the quantitative analysis of oleiferasaponin A1 using LC/UV was developed. The purified total saponins did not exhibit any inhibitory effects at concentrations ranging from 0.1 to 10 mg/mL against the tested bacteria, except for Staphyloccocus aureus and Escherichia coli. By contrast, higher inhibitory activity was seen against the tested fungi, especially against Bipolaris maydis. Following treatment with an MIC value of 250 μg/mL for 24 h, the mycelial morphology was markedly shriveled in appearance or showed flattened and empty hyphae, with fractured cell walls, ruptured plasmalemma and cytoplasmic coagulation or leakage. These structural changes hindered the growth of mycelia.

建立了一种使用 LC-ESI-IT-TOF/MS 和 LC/UV-ELSD 的方法，用于定性分析从茶籽饼（Camellia oleifera Abel）中获得的三萜皂苷。此外，还开发了使用 LC/UV 对 oleiferasaponin A1 进行定量分析的方法。在 0.1 至 10 mg/mL 的浓度范围内，纯化的总皂苷对测试的细菌（除金黄色葡萄球菌和大肠杆菌外）没有任何抑制作用。相比之下，它对测试的真菌表现出更高的抑制活性，尤其是对玉米黑粉菌。用 MIC 值为 250 μg/mL 处理 24 h 后，菌丝体形态明显萎缩，或表现出压扁和空的菌丝，细胞壁破裂，质膜破裂，细胞质凝结或渗漏。这些结构变化阻碍了菌丝体的生长。