Webster-Cyriaque J, Edwards R H, Quinlivan E B, Patton L, Wohl D, Raab-Traub N
Department of Microbiology and Immunology and Lineberger Cancer Center, University of North Carolina at Chapel Hill, 27599-7295, USA.
J Infect Dis. 1997 Jun;175(6):1324-32. doi: 10.1086/516463.
The prevalence of Epstein-Barr virus (EBV) and the recently identified Kaposi's sarcoma (KS)-associated herpesvirus (also designated human herpesvirus 8 [HHV-8]) was determined in oral lesions and oral neoplasms common to persons with human immunodeficiency virus (HIV) infection. Oral lesions were examined by polymerase chain reaction (PCR) for EBV and HHV-8 DNA and by Southern blot analysis for EBV clonality. EBV was detected by Southern blot in hairy leukoplakia lesions, in a subset of AIDS-related lymphomas, and in saliva from HIV-positive persons but not in pseudohairy leukoplakia lesions, oral aphthous ulcers, or oral KS lesions. EBV was detected, however, by PCR in most of the lesions, while HHV-8 was detected only in oral KSs. The absence of HHV-8 DNA in both the EBV-associated hairy leukoplakia lesions and in the EBV-associated AIDS-related lymphomas strengthens the etiologic relationship of EBV to these pathologies and the etiologic role of HHV-8 in KS.
在人类免疫缺陷病毒（HIV）感染者常见的口腔病变和口腔肿瘤中，测定了爱泼斯坦-巴尔病毒（EBV）和最近发现的卡波西肉瘤（KS）相关疱疹病毒（也称为人类疱疹病毒8型[HHV-8]）的流行情况。通过聚合酶链反应（PCR）检测口腔病变中的EBV和HHV-8 DNA，并通过Southern印迹分析检测EBV的克隆性。通过Southern印迹在毛状白斑病变、一部分与艾滋病相关的淋巴瘤以及HIV阳性者的唾液中检测到EBV，但在假性毛状白斑病变、口腔阿弗他溃疡或口腔KS病变中未检测到。然而，通过PCR在大多数病变中检测到EBV，而仅在口腔KS中检测到HHV-8。在与EBV相关的毛状白斑病变和与EBV相关的艾滋病相关淋巴瘤中均未检测到HHV-8 DNA，这强化了EBV与这些病变的病因学关系以及HHV-8在KS中的病因学作用。
