Morisaki N, Saito I, Tamura K, Tashiro J, Masuda M, Kanzaki T, Watanabe S, Masuda Y, Saito Y
The Second Department of Internal Medicine, School of Medicine, Chiba University, Chuo-ku, Japan.
Atherosclerosis. 1997 May;131(1):43-8. doi: 10.1016/s0021-9150(97)06083-8.
It is known that the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on the surface of vascular endothelial cells is closely related to the formation of early atherosclerotic lesions. In this study, serum soluble ICAM-1(sICAM-1) and soluble VCAM-1(sVCAM-1) were determined by sandwich ELISA both in normal healthy individuals (n = 114) and in patients with hypercholesterolemia (HC, n = 112) or ischemic heart disease (IHD, n = 38) to clarify the significance of the soluble forms of the adhesion molecules in the development of atherosclerotic diseases. IHD patients, not HC patients, showed significant elevation of sICAM-1, but not of sVCAM-1, compared with controls in age and sex-matched subjects. In addition, multiple linear regression analysis showed that sICAM-1 was correlated only to the presence of IHD but not to age and lipids. Multiple logistic regression analysis revealed that sICAM-1 was the most powerful independent predictor of the presence of IHD. On the other hand, sVCAM-1, not sICAM-1, was positively correlated to age. Multiple linear regression analysis showed that age was the most powerful independent predictor of the level of sVCAM-1. These data suggest that sICAM-1 and sVCAM-1 are useful as indices of clinical manifestations of atherosclerosis and aging, respectively.
已知血管内皮细胞表面细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的表达与早期动脉粥样硬化病变的形成密切相关。在本研究中，采用夹心ELISA法测定了正常健康个体（n = 114）、高胆固醇血症患者（HC，n = 112）或缺血性心脏病患者（IHD，n = 38）血清中的可溶性ICAM-1（sICAM-1）和可溶性VCAM-1（sVCAM-1），以阐明黏附分子可溶性形式在动脉粥样硬化疾病发展中的意义。与年龄和性别匹配的对照组相比，IHD患者而非HC患者的sICAM-1显著升高，但sVCAM-1未升高。此外，多元线性回归分析表明，sICAM-1仅与IHD的存在相关，而与年龄和血脂无关。多元logistic回归分析显示，sICAM-1是IHD存在的最有力独立预测因子。另一方面，sVCAM-1而非sICAM-1与年龄呈正相关。多元线性回归分析表明，年龄是sVCAM-1水平的最有力独立预测因子。这些数据表明，sICAM-1和sVCAM-1分别可作为动脉粥样硬化和衰老临床表现的指标。
