Kotelkin A T, Razumov I A, Pokrovskiĭ I V, Loktev V B
Med Parazitol (Mosk). 1997 Jan-Mar(1):12-6.
Electrophoretic analysis showed that the somatic, excretory and secretory, and egg antigens of O. felineus have a complex protein composition. They were found to have 20, 11, and 25 proteins, respectively. On titration of the antigens, by using a constant dose of immune sera, the excretory and secretory antigen was detected in concentrations 50-100 less than the somatic and egg antigens. Rabbit immune sera effectively interacted with the proteins 116, 105, 80, 70, 60, 50, 44, 40 kD as part of the excretory and secretory antigen, with the proteins 105, 80, 70, 60, 50, 44, 40 and 32 kD as part of the somatic antigen, with the proteins p70, p60, p50, p28, p25 and p24 kD as part of the egg one. The limited human immune responses revealed by the author on O. felineus invasion, which was associated with the excretory and secretory and somatic p105 and three egg antigen proteins, such as p74, p70, p64 clearly indicate further ways of improving the immune diagnosis of opisthorchiasis.
电泳分析表明，猫后睾吸虫的体抗原、排泄及分泌抗原和虫卵抗原有复杂的蛋白质组成。它们分别含有20、11和25种蛋白质。在用恒定剂量的免疫血清滴定抗原时，检测到排泄及分泌抗原的浓度比体抗原和虫卵抗原低50 - 100倍。兔免疫血清能有效与排泄及分泌抗原中的116、105、80、70、60、50、44、40 kD蛋白质相互作用，与体抗原中的105、80、70、60、50、44、40和32 kD蛋白质相互作用，与虫卵抗原中的p70、p60、p50、p28、p25和p24 kD蛋白质相互作用。作者发现人体对猫后睾吸虫感染的免疫反应有限，这与排泄及分泌抗原、体抗原p105以及三种虫卵抗原蛋白（如p74、p70、p64）有关，这清楚地表明了进一步改进华支睾吸虫病免疫诊断方法的途径。
