Poole C A, Ayad S, Schofield J R
Department of Surgery, University of Auckland, School of Medicine, New Zealand.
J Cell Sci. 1988 Aug;90 ( Pt 4):635-43. doi: 10.1242/jcs.90.4.635.
A heterogenous population of intact chondrons extracted from low-speed homogenates of canine tibial cartilage were stained by indirect immunofluorescence methods with a polyclonal antibody to type VI collagen. In each of the four chondron groups examined, anti-(type VI collagen) anti-serum was concentrated in the capsule immediately adjacent to the chondrocyte complex. A constant but weaker fluorescent reaction persists in 'tail-like' extensions common to single and double chondrons and in the medial connections between adjacent chondrons in linear columns and aggregated clusters. Frayed collagen bundles typical of chondron preparations did not react with the antibody. Similarly, chondrons reacted with normal rabbit serum, or treated by type VI collagen extraction procedures, showed no staining reaction. The differential localization of type VI collagen in the pericellular capsule is discussed in relation to the maintenance of the chondron's integrity and to the protection of the chondrocyte during dynamic compressive loading.
从犬胫骨软骨低速匀浆中提取的完整软骨粒异质群体，用抗VI型胶原多克隆抗体通过间接免疫荧光法进行染色。在检查的四个软骨粒组中的每一组中，抗（VI型胶原）抗血清都集中在紧邻软骨细胞复合体的囊膜中。在单个和双个软骨粒共有的“尾状”延伸部分以及线性柱和聚集簇中相邻软骨粒之间的内侧连接中，持续存在恒定但较弱的荧光反应。软骨粒制剂中典型的松解胶原束不与抗体发生反应。同样，用正常兔血清处理或通过VI型胶原提取程序处理的软骨粒也没有染色反应。讨论了VI型胶原在细胞周围囊膜中的差异定位与软骨粒完整性的维持以及动态压缩载荷期间软骨细胞保护的关系。
