Srivastava P K
Department of Biological Sciences, Fordham University, Bronx, New York 10458, USA.
Methods. 1997 Jun;12(2):165-71. doi: 10.1006/meth.1997.0464.
This article describes the methods used for the purification of heat shock proteins gp96, hsp90, and hsp70 from murine and human tissues and cell lines. The heat shock proteins purified by the methods described are associated noncovalently with an array of cellular peptides. The use of heat shock protein-peptide complexes for eliciting prophylactic and therapeutic immunity to cancers and infectious diseases is discussed.
本文描述了从鼠类和人类组织及细胞系中纯化热休克蛋白gp96、hsp90和hsp70的方法。通过所述方法纯化的热休克蛋白与一系列细胞肽非共价结合。文中还讨论了热休克蛋白-肽复合物在引发针对癌症和传染病的预防性及治疗性免疫方面的应用。
