Hansson M, Eriksson A, Forsgren S
Department of Anatomy, Umeå University, Sweden.
Histochem J. 1997 Apr;29(4):329-36. doi: 10.1023/a:1026478616039.
Atrial natriuretic peptide is a well-described peptide in cardiac Purkinje fibres and has been shown to interfere with the autonomic regulation in the heart of various species, including man. Recently, we detected immunoreactivity for the peptide in intracardial ganglionic cells and nerve fibre varicosities of bovine hearts, by the use of a modified immunostaining technique that induced an improved detection of natriuretic peptides. These findings raised the question as to whether natriuretic peptides are detectable in these tissues in man and other species. The conduction system from human, pig and sheep hearts was dissected processed with antisera against atrial natriuretic peptide and the closely related brain natriuretic peptide. Immunostaining for the brain natriuretic peptide was detected in some Purkinje fibres in all of these species. Interestingly, in pig, sheep and human hearts, some ganglionic cells and nerve fibres showed atrial natriuretic peptide immunoreactivity, particularly in the soma of human ganglionic cells. This is the first study showing immunoreactivity for the atrial natriuretic peptide in nerve structures and for the brain natriuretic peptide in Purkinje fibres of the human heart. The results give a morphological correlate for the documented effects of atrial natriuretic peptide on the heart autonomic nervous system and for the presumable effects of brain natriuretic peptide in the conduction system of man.
心房利钠肽是一种在心脏浦肯野纤维中已被充分描述的肽，并且已被证明会干扰包括人类在内的各种物种心脏中的自主调节。最近，我们通过使用一种改良的免疫染色技术，在牛心脏的心内神经节细胞和神经纤维膨体中检测到了该肽的免疫反应性，这种技术提高了利钠肽的检测率。这些发现引发了一个问题，即利钠肽在人类和其他物种的这些组织中是否可被检测到。我们解剖了人类、猪和绵羊心脏的传导系统，并用抗心房利钠肽和密切相关的脑利钠肽的抗血清进行处理。在所有这些物种的一些浦肯野纤维中检测到了脑利钠肽的免疫染色。有趣的是，在猪、绵羊和人类心脏中，一些神经节细胞和神经纤维显示出心房利钠肽免疫反应性，特别是在人类神经节细胞的胞体中。这是第一项显示人类心脏神经结构中心房利钠肽免疫反应性以及浦肯野纤维中脑利钠肽免疫反应性的研究。这些结果为心房利钠肽对心脏自主神经系统的已记录效应以及脑利钠肽在人类传导系统中的推测效应提供了形态学关联。
