Donald J A, Evans D H
Department of Zoology, University of Florida, Gainesville 32611.
Cell Tissue Res. 1992 Jul;269(1):151-8. doi: 10.1007/BF00384735.
The distribution of natriuretic peptide immunoreactivity was determined in the heart and brain of the gulf toadfish Opsanus beta using the avidin-biotin peroxidase technique. Four antisera were used: the first raised against porcine brain natriuretic peptide which cross-reacts with atrial natriuretic and C-type natriuretic peptides (termed natriuretic peptide-like immunoreactivity); the second raised against porcine brain natriuretic peptide which cross-reacts with C-type natriuretic peptide but not with atrial natriuretic peptide (termed porcine brain natriuretic peptide-like immunoreactivity); the third raised against rat atrial natriuretic peptide; and the fourth raised against eel atrial natriuretic peptide. Natriuretic peptide- and porcine brain natriuretic peptide-like immunoreactivity was observed in all cardiac muscle cells of the atrium. In the ventricle, natriuretic peptide-like immunoreactivity was found in all cardiac muscle cells, however, porcine brain natriuretic peptide-like immunoreactivity was confined to muscle cells adjacent to the epicardium. There was no discernible difference in the distribution of natriuretic peptide-like immunoreactivity and porcine brain natriuretic peptide-like immunoreactivity in the brain. Immunoreactive perikarya were observed only in the preoptic region of the diencephalon, and many immunoreactive fibres were found in the telencephalon, preoptic area, and rostral hypothalamus, lateral to the thalamic region. There was no immunoreactivity in any region of the hypophysis. A pair of distinct immunoreactive fibre tracts ran caudally from the preoptic area to the thalamic region, from which fibres extended to the posterior commissure, area praetectalis, dorsolateral regions of the midbrain tegmentum, and tectum.(ABSTRACT TRUNCATED AT 250 WORDS)
采用抗生物素蛋白-生物素过氧化物酶技术，测定了海湾蟾鱼（Opsanus beta）心脏和脑中利钠肽免疫反应性的分布。使用了四种抗血清：第一种是针对猪脑利钠肽产生的，它与心房利钠肽和C型利钠肽发生交叉反应（称为利钠肽样免疫反应性）；第二种是针对猪脑利钠肽产生的，它与C型利钠肽发生交叉反应，但不与心房利钠肽发生交叉反应（称为猪脑利钠肽样免疫反应性）；第三种是针对大鼠心房利钠肽产生的；第四种是针对鳗鱼心房利钠肽产生的。在心房的所有心肌细胞中均观察到利钠肽和猪脑利钠肽样免疫反应性。在心室中，所有心肌细胞中均发现利钠肽样免疫反应性，然而，猪脑利钠肽样免疫反应性仅限于心外膜附近的肌肉细胞。脑中利钠肽样免疫反应性和猪脑利钠肽样免疫反应性的分布没有明显差异。仅在间脑的视前区观察到免疫反应性核周体，并且在端脑、视前区和丘脑区域外侧的 Rostral 下丘脑发现了许多免疫反应性纤维。垂体的任何区域均无免疫反应性。一对明显的免疫反应性纤维束从视前区向尾侧延伸至丘脑区域，纤维从该区域延伸至后连合、前检测区、中脑被盖背外侧区域和顶盖。（摘要截断于250字）
