Nakajima A, Watanabe N, Yoshino S, Yagita H, Okumura K, Azuma M
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Int Immunol. 1997 May;9(5):637-44. doi: 10.1093/intimm/9.5.637.
The interaction between CD28 and its ligands, CD80 and CD86, is crucial for an optimal activation of antigen-specific T cells. However, the requirement of CD80 or CD86 co-stimulation in Th2 cell differentiation and activation is controversial. Freshly isolated murine CD4+ and CD8+ T cells were incubated with P815 transfectants expressing a similar level of either CD80 or CD86 in the presence of anti-CD3 mAb. Both CD80 and CD86 co-stimulated the proliferation of CD4+ and CD8+ T cells at comparable time-kinetics and magnitude, but CD86 alone was able to co-stimulate IL-4 and especially IL-10 production in CD4+ T cells. In typical Th2-dependent immune responses elicited by Nippostrongylus brasillensis infection, the anti-CD86 mAb treatment but not the anti-CD80 mAb treatment efficiently inhibited antigen-specific IgE and IgG1 production, which was accompanied with the reduced IL-4 production. Our results suggest that CD86 co-stimulation plays a dominant role not only in the primary activation of Th2 cells but also in the secondary interaction between antigen-primed Th2 cells and B cells.
CD28与其配体CD80和CD86之间的相互作用对于抗原特异性T细胞的最佳激活至关重要。然而，CD80或CD86共刺激在Th2细胞分化和激活中的必要性存在争议。将新鲜分离的小鼠CD4+和CD8+ T细胞与在抗CD3单克隆抗体存在下表达相似水平的CD80或CD86的P815转染细胞一起孵育。CD80和CD86均以相当的时间动力学和幅度共刺激CD4+和CD8+ T细胞的增殖，但单独的CD86能够共刺激CD4+ T细胞产生IL-4，尤其是IL-10。在由巴西日圆线虫感染引发的典型Th2依赖性免疫反应中，抗CD86单克隆抗体处理而非抗CD80单克隆抗体处理有效抑制了抗原特异性IgE和IgG1的产生，同时伴随着IL-4产生的减少。我们的结果表明，CD86共刺激不仅在Th2细胞的初次激活中起主导作用，而且在抗原致敏的Th2细胞与B细胞之间的二次相互作用中也起主导作用。
