编码旋毛虫肌幼虫抗原及其来自山猫源旋毛虫T5类似物的互补DNA：序列、克隆与表达

A complementary DNA encoding an antigen from Trichinella spiralis muscle larvae and its analog from Trichinella T5 of bobcat origin: sequence, cloning and expressions.

作者信息

Yao C, McGraw R A, Prestwood A K

机构信息

Department of Parasitology, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.

出版信息

Int J Parasitol. 1997 Apr;27(4):425-30. doi: 10.1016/s0020-7519(97)00001-5.

DOI:10.1016/s0020-7519(97)00001-5

PMID:9184935

Abstract

Reverse transcription-polymerase chain reaction (RT-PCR) was employed to amplify a cDNA encoding an excretory-secretory (ES) antigen with mol. wt 45-50 kDa by SDS-PAGE from T. spiralis muscle larvae. The PCR product was purified by electrophoresis and sequenced by thermal cycle sequencing with primer walking. The cDNA is 890 bp long and encodes a polypeptide of 255 amino acid (AA) residues. Using the same methods, we also recovered a corresponding cDNA from Trichinella T5, which is 891 bp long and encodes 255 AAs. Comparison of the 2 Trichinella species indicates approximately 2.6% and 2.4% differences between the 2 cDNA sequences and between the 2 deduced AA sequences, respectively.

摘要

采用逆转录聚合酶链反应（RT-PCR）从旋毛虫肌幼虫中扩增出一个编码排泄分泌（ES）抗原的cDNA，该抗原经SDS-PAGE分析分子量为45-50 kDa。PCR产物经电泳纯化，采用引物步移法进行热循环测序。该cDNA长890 bp，编码一个由255个氨基酸（AA）残基组成的多肽。使用相同方法，我们还从旋毛虫T5中获得了一个相应的cDNA，其长891 bp，编码255个AA。两种旋毛虫的比较表明，两个cDNA序列之间以及两个推导的AA序列之间分别存在约2.6%和2.4%的差异。