Yao C, Prestwood A K, McGraw R A
Department of Parasitology, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
J Parasitol. 1997 Feb;83(1):88-95.
We compared Trichinella T5 of bobcat (Lynx rufus) origin with Trichinella spiralis (T1) by using animal infectivity and molecular biology techniques. Swine, SD rats, and CF1 mice were highly resistant to infection with Trichinella T5 but sensitive to T. spiralis, whereas deer mice (peromyscus maniculatus) had similar sensitivity to both parasites. The fecundity of Trichinella T5 in deer mice was 10-35-fold higher in comparison to the fecundity in laboratory rodents (SD rats and CF1 mice). Fecundity of T. spiralis was approximately the same in both groups. A western blot, using excretory-secretory proteins (ESP) from first-stage larvae of T. spiralis as antigen, showed similar banding patterns in the pigs infected with either T. spiralis or Trichinella T5, however, the homologous reaction was stronger than the heterologous reaction. Antibodies were detectable in swine sera commencing 3 or 5 wk postinfection with T. spiralis or Trichinella T5, respectively. Complementary DNAs encoding the 46-, 49/43-, or 53-kDa ESP showed 3.54, 1.94, and 5.91% differences, respectively, between the 2 parasites. Deduced amino acid sequences of the 3 cDNAs were different at 7.20, 5.08, and 8.55%, respectively. All recombinant proteins of the 3 cDNAs from both parasites could detect antibodies in positive sera. The sequences of cDNAs encoding the 46-, 49/43-, or 53-kDa ESP from T. spiralis are also compared to the previously reported sequences, and the differences are discussed.
我们运用动物感染性和分子生物学技术，将源自山猫（短尾猫）的旋毛虫T5与旋毛形线虫（T1）进行了比较。猪、SD大鼠和CF1小鼠对旋毛虫T5感染具有高度抗性，但对旋毛形线虫敏感，而鹿鼠（白足鼠）对这两种寄生虫的敏感性相似。与实验室啮齿动物（SD大鼠和CF1小鼠）相比，旋毛虫T5在鹿鼠体内的繁殖力高10至35倍。旋毛形线虫在两组中的繁殖力大致相同。以旋毛形线虫一期幼虫的排泄分泌蛋白（ESP）为抗原进行的蛋白质印迹分析表明，感染旋毛形线虫或旋毛虫T5的猪呈现出相似的条带模式，然而，同源反应强于异源反应。分别在感染旋毛形线虫或旋毛虫T5后3周或5周，猪血清中可检测到抗体。编码46 kDa、49/43 kDa或53 kDa ESP的互补DNA在这两种寄生虫之间分别显示出3.54%、1.94%和5.91%的差异。这3个cDNA推导的氨基酸序列差异分别为7.20%、5.08%和8.55%。两种寄生虫的这3个cDNA的所有重组蛋白均可在阳性血清中检测到抗体。还将旋毛形线虫编码46 kDa、49/43 kDa或53 kDa ESP的cDNA序列与先前报道的序列进行了比较，并讨论了差异。
