Wang I J, Shih Y F, Shih B C, Huang S H, Lin L L, Hung P T
Department of Ophthalmology, National Taiwan University Hospital, Taipei, Taiwan.
J Ocul Pharmacol Ther. 1997 Jun;13(3):253-60. doi: 10.1089/jop.1997.13.253.
The mechanism of axial elongation caused by experimental or clinical myopia is still unknown. We sought to explore the changes of scleral chondrocytes during myopia formation through the cell biology model. White Leghorn chicks were used for this study. The right eye was covered with a translucent goggle after hatching, and the left eye was left uncovered for control. The chicks were maintained on 12 hours light-dark cycle for two weeks, then sacrificed every other day and the eyeballs removed for study. Our results in the primary culture of scleral chondrocytes showed that the densities of chondrocytes on myopic eyes were significantly higher than those of the controlled non-myopic eyes, and 3H-thymidine incorporation rate also increased with the increasing of the concentration of fetal bovine serum. The PCNA index of chondrocytes in myopic eyes was also higher than that of the controlled non-myopic eyes. Thus, axial elongation of experimental myopia in the chick is the result of active tissue remodeling rather than passive scleral stretching alone.
实验性或临床性近视所导致的眼轴伸长机制仍不清楚。我们试图通过细胞生物学模型来探究近视形成过程中巩膜软骨细胞的变化。本研究使用了白来航鸡。雏鸡孵化后，右眼用半透明眼罩遮盖，左眼不遮盖作为对照。雏鸡维持12小时光照 - 黑暗循环两周，然后每隔一天处死，取出眼球进行研究。我们在巩膜软骨细胞原代培养中的结果显示，近视眼中软骨细胞的密度显著高于对照的非近视眼中的软骨细胞密度，并且3H - 胸腺嘧啶核苷掺入率也随着胎牛血清浓度的增加而升高。近视眼中软骨细胞的增殖细胞核抗原（PCNA）指数也高于对照的非近视眼。因此, 雏鸡实验性近视的眼轴伸长是活跃的组织重塑的结果，而不仅仅是巩膜被动拉伸的结果。
