Owhashi M, Kirai N, Asami M
Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Southeast Asian J Trop Med Public Health. 1996 Sep;27(3):530-4.
Infection of mice with Plasmodium berghei engendered a temporary appearance of granulocyte-macrophage colony-stimulating factor (GM-CSF) in the serum. The peak of GM-CSF levels was detected at day 2 post-infection, and then gradually decreased. On the other hand, the number of committed stem cells for granulocytes and macrophages (CFU-GM) in bone marrow transiently decreased at day 2 post-infection, and then increased and peaked at day 6 post-infection. When the serum of P. berghei-infected mice was fractionated by gel chromatography on Sephacryl S-300, GM-CSF activity was detected as a single peak with an apparent molecular weight of 64 KDa. GM-CSF was entirely adsorbed to concanavalin A-Sepharose 4B affinity chromatography, and was sensitive to pronase digestion, indicating its glycoprotein nature. These results suggest that the circulating GM-CSF would contribute the increase of granulocyte-macrophage hemopoiesis in the early phase of malaria.
用伯氏疟原虫感染小鼠会使血清中暂时出现粒细胞巨噬细胞集落刺激因子（GM-CSF）。GM-CSF水平在感染后第2天达到峰值，然后逐渐下降。另一方面，骨髓中粒细胞和巨噬细胞定向干细胞（CFU-GM）的数量在感染后第2天短暂减少，然后增加并在感染后第6天达到峰值。当用Sephacryl S-300凝胶色谱法对感染伯氏疟原虫的小鼠血清进行分级分离时，GM-CSF活性被检测为一个单一峰，表观分子量为64 kDa。GM-CSF完全吸附到伴刀豆球蛋白A-琼脂糖4B亲和色谱上，并且对链霉蛋白酶消化敏感，表明其糖蛋白性质。这些结果表明，循环中的GM-CSF会在疟疾早期促进粒细胞巨噬细胞造血的增加。
