Owhashi M, Uemura H, Kanbara H, Nawa Y
Faculty of Integrated Arts and Sciences, University of Tokushima, Japan.
Southeast Asian J Trop Med Public Health. 1997 Dec;28(4):757-63.
The production of granulocyte-macrophage colony-stimulating factor (GM-CSF) by lymphocytes was examined in murine malaria. When spleen cells or lymph node cells from P. berghei-infected mice were cultured in vitro with malaria antigen, the GM-CSF production correlated with the incubation time up to 72 hours. When lymphocytes obtained at various days after infection were cultured with the antigen, GM-CSF became detectable as early as 2 days after infection, reached a peak at day 9 and then rapidly decreased. Production of GM-CSF was antigen-specific, and related to the dose of antigen. Treatment of lymphocytes with anti-Thy-1.2 antibody and complement resulted in almost complete loss of GM-CSF-producing activity, while treatment with either anti-CD4 or anti-CD8 antibody and complement resulted in partial loss of GM-CSF-producing activity, indicating that both CD4+ and CD8+ T cells are involved in GM-CSF production in malaria. GM-CSF exhibits glycoprotein nature, and has an apparent molecular weight of 36,000. The molecular properties of this T-cell derived GM-CSF were compared with those of known lymphokine GM-CSF.
在鼠疟模型中检测了淋巴细胞产生粒细胞-巨噬细胞集落刺激因子（GM-CSF）的情况。当将来自感染伯氏疟原虫小鼠的脾细胞或淋巴结细胞与疟疾抗原在体外培养时，GM-CSF的产生与长达72小时的孵育时间相关。当用抗原培养在感染后不同天数获得的淋巴细胞时，GM-CSF最早在感染后2天即可检测到，在第9天达到峰值，然后迅速下降。GM-CSF的产生具有抗原特异性，且与抗原剂量有关。用抗Thy-1.2抗体和补体处理淋巴细胞会导致GM-CSF产生活性几乎完全丧失，而用抗CD4或抗CD8抗体和补体处理则会导致GM-CSF产生活性部分丧失，这表明CD4⁺和CD8⁺ T细胞均参与疟疾中GM-CSF的产生。GM-CSF具有糖蛋白性质，表观分子量为36,000。将这种T细胞来源的GM-CSF的分子特性与已知的淋巴因子GM-CSF的分子特性进行了比较。
