Morgan M B, Pitha J, Johnson S, Dunn B, Everett M A
Department of Dermatology, University of Oklahoma Medical Centers, Oklahoma City 73014, USA.
Am J Dermatopathol. 1997 Jun;19(3):223-7. doi: 10.1097/00000372-199706000-00004.
A histologic, immunohistochemical, and DNA ploidy analyses were performed on two cases of angiomatoid malignant fibrous histiocytoma to ascertain the histogenesis and relationship of endothelial, histiocytic, and fibroblastic elements. Both cases were slowly growing, grossly encapsulated. Subcutaneous masses resected from pediatric patients. Microscopically, the tumors were composed of solid masses of epithelioid and spindle cells with abnormal endothelial-lined and blood-filled cystic spaces surrounded by normal vascular structures and aggregates of lymphocytes occasionally forming germinal follicles. The tumor cells stained exclusively with CD34 and vimentin antibodies. Tumor-associated vessels stained for CD31, CD34, vimentin, and Ulex europaeus. Occasional cells within germinal follicles stained for lysozyme, CD68, and HAM56. Ploidy analysis of tumor cells showed intermediate aneuploidy with a DNA index of 1.14. Blood vessels within and surrounding the tumor as well as inflammatory cells were DNA euploid. These studies suggest that the tumor--though comprised of histologically and immunohistochemically benign-appearing euploid endothelial, fibroblastic, and inflammatory elements--contains an aneuploid population of undifferentiated mesenchymal cells.
对两例血管样恶性纤维组织细胞瘤进行了组织学、免疫组织化学及DNA倍体分析，以确定其组织发生以及内皮细胞、组织细胞和成纤维细胞成分之间的关系。两例均生长缓慢，肉眼可见包膜。肿瘤取自儿科患者的皮下肿块。显微镜下，肿瘤由上皮样细胞和梭形细胞的实性肿块组成，伴有异常的内衬内皮细胞和充满血液的囊性间隙，周围有正常血管结构以及偶尔形成生发滤泡的淋巴细胞聚集。肿瘤细胞仅对CD34和波形蛋白抗体染色呈阳性。肿瘤相关血管对CD31、CD34、波形蛋白和荆豆凝集素染色呈阳性。生发滤泡内的偶尔细胞对溶菌酶、CD68和HAM56染色呈阳性。肿瘤细胞的倍体分析显示为中度非整倍体，DNA指数为1.14。肿瘤内部及周围的血管以及炎性细胞为DNA整倍体。这些研究表明，该肿瘤——尽管由组织学和免疫组织化学上看似良性的整倍体内皮细胞、成纤维细胞和炎性细胞组成——含有一群非整倍体的未分化间充质细胞。
