Marsh B J, Fordham von Reyn C, Arbeit R D, Morin P
Infectious Disease Section, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, USA.
Am J Med Sci. 1997 Jun;313(6):377-83. doi: 10.1097/00000441-199706000-00011.
Heat-killed Mycobacterium vaccae vaccine was administered in a 3-dose schedule to 12 HIV-infected adults with CD4 cell counts > or = 300/mm3. Local and systemic side effects were monitored. Delayed-type hypersensitivity to purified protein derivative and Mycobacterium avium sensitin was measured at baseline and after the final dose. Antibody to aralipoarabinomannin, man-lipoarabinomannin, and a short-term culture filtrate of Mycobacterium tuberculosis were also measured. Lymphocyte proliferation responses to M avium sensitin and M vaccae sonicate were determined. Vaccine site induration was maximal at 2 days (median, 6 mm) and no systemic side effects were noted. Purified protein derivative skin test conversions did not occur. Changes in CD4 counts and HIV viral load were not significant. Three (27%) of 11 subjects who completed the trial showed either M avium skin test (n = 1) or short-term culture filtrate antibody (n = 2) responses. A three-dose schedule of M vaccae vaccine is safe and well tolerated in adults with early HIV infection and produces detectable immunologic responses in a subset of these subjects.
对12名CD4细胞计数≥300/mm³的HIV感染成人按3剂方案接种热灭活母牛分枝杆菌疫苗。监测局部和全身副作用。在基线和最后一剂后测量对纯化蛋白衍生物和鸟分枝杆菌敏感素的迟发型超敏反应。还测量了抗阿拉伯糖脂甘露聚糖、甘露糖-阿拉伯糖脂甘露聚糖和结核分枝杆菌短期培养滤液的抗体。测定对鸟分枝杆菌敏感素和母牛分枝杆菌超声裂解物的淋巴细胞增殖反应。疫苗接种部位硬结在第2天最大（中位数为6mm），未观察到全身副作用。纯化蛋白衍生物皮肤试验未出现阳转。CD4计数和HIV病毒载量的变化不显著。完成试验的11名受试者中有3名（27%）出现了对鸟分枝杆菌皮肤试验（n = 1）或短期培养滤液抗体（n = 2）反应。三剂方案的母牛分枝杆菌疫苗在早期HIV感染成人中安全且耐受性良好，并在这些受试者的一部分中产生可检测到的免疫反应。
