von Reyn C F, Marsh B J, Waddell R, Lein A D, Tvaroha S, Morin P, Modlin J F
Infectious Disease Section, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire 03756, USA.
Clin Infect Dis. 1998 Dec;27(6):1517-20. doi: 10.1086/515024.
The safety and immunogenicity of heat-killed Mycobacterium vaccae vaccine were investigated in a pilot study assessing the feasibility of immunization to prevent mycobacterial disease in patients with human immunodeficiency virus (HIV) infection. Fifteen (seven healthy and eight HIV-positive subjects) received five doses of M. vaccae vaccine. Lymphocyte proliferation assays (LPAs) were performed using Mycobacterium avium sensitin (MAS) and M. vaccae sonicate (MVS). Vaccine was well tolerated in all 15 subjects with minimal induration at the vaccine site. LPAs for four of seven healthy vaccines were positive for MAS after immunization. Median responses to MAS and MVS that were determined by LPAs were consistently higher for the eight HIV-positive vaccinees than for the seven healthy controls. A five-dose series of M. vaccae vaccine is safe for both healthy and HIV-positive subjects and deserves further evaluation as a vaccine to prevent HIV-associated mycobacterial disease.
在一项初步研究中，对热灭活母牛分枝杆菌疫苗的安全性和免疫原性进行了调查，该研究评估了免疫接种预防人类免疫缺陷病毒（HIV）感染患者分枝杆菌病的可行性。15名受试者（7名健康受试者和8名HIV阳性受试者）接受了5剂母牛分枝杆菌疫苗。使用鸟分枝杆菌致敏原（MAS）和母牛分枝杆菌超声裂解物（MVS）进行淋巴细胞增殖试验（LPA）。所有15名受试者对疫苗耐受性良好，疫苗接种部位硬结极小。7名健康疫苗接种者中有4名在免疫后对MAS的LPA呈阳性。通过LPA测定，8名HIV阳性疫苗接种者对MAS和MVS的中位反应始终高于7名健康对照者。5剂系列的母牛分枝杆菌疫苗对健康受试者和HIV阳性受试者均安全，作为预防HIV相关分枝杆菌病的疫苗值得进一步评估。
