Wagner T, Tommerup N, Wirth J, Leffers H, Zimmer J, Back E, Weissenbach J, Scherer G
Institute of Human Genetics and Anthropology, University of Freiburg, Germany.
Cytogenet Cell Genet. 1997;76(3-4):172-5. doi: 10.1159/000134538.
A somatic cell hybrid panel was constructed consisting of seven hybrids with translocation breakpoints spanning the region 17q23-->q25. Hybrid clones carrying the longarm derivative of chromosome 17 in the absence of the normal chromosome 17 and of the derivative 17 were initially identified by PCR typing for a proximal and distal 17q marker. The translocation breakpoints of the hybrids were then mapped in more detail by PCR analysis for a number of microsatellite markers from chromosome 17q as well as for five gene loci (CACNLG, GH1, SOX9, TIMP2, TK1) previously mapped to the region 17q23-->q25. In addition, the locus for GDIA1 was mapped by FISH to 17q25.3 and fine mapped with the help of the hybrid panel. These seven new hybrids complement the existing somatic cell hybrid panel for the long arm of chromosome 17q.
构建了一个体细胞杂交板，由七个杂交体组成，其易位断点跨越17q23→q25区域。最初通过对近端和远端17q标记进行PCR分型，鉴定出携带17号染色体长臂衍生物而无正常17号染色体和17号衍生物的杂交克隆。然后，通过对来自17q染色体的多个微卫星标记以及先前定位于17q23→q25区域的五个基因座（CACNLG、GH1、SOX9、TIMP2、TK1）进行PCR分析，更详细地绘制杂交体的易位断点图。此外，通过荧光原位杂交（FISH）将GDIA1基因座定位于17q25.3，并借助杂交板进行精细定位。这七个新的杂交体补充了现有的17q染色体长臂体细胞杂交板。
