免疫过氧化物酶单层试验在虫媒病毒抗体检测中的应用。
Application of an immunoperoxidase monolayer assay for the detection of arboviral antibodies.
作者信息
Soliman A K, Watts D M, Salib A W, Shehata A E, Arthur R R, Botros B A
机构信息
Virology Branch, US Naval Medical Research Unit No. 3. Cairo, Egypt.
出版信息
J Virol Methods. 1997 May;65(2):147-51.
An immunoperoxidase monolayer assay (IPMA) was adapted for the detection of antibodies to six arboviruses: three viruses within the flavivirus group (dengue 2, West Nile (WN) and yellow fever) and three in the phlebovirus group (Rift Valley fever (RVF), sandfly fever Naples and sandfly fever Sicilian). Antibody titers of homologous hyper-immune mouse ascitic fluid (HMAF) measured by IPMA were two to eight-fold less than those determined by ELISA. In tests with heterologous HMAF, cross-reactions frequently observed in ELISA, particularly in the flavivirus group, were absent in all IPMA titrations. With human serum samples tested for antibodies to RVF (n = 52) and WN (n = 90), the sensitivity of IPMA as compared with ELISA was 96 and 91%, respectively, specificity of IPMA was 100%. In addition, the IPMA format has several advantages that make it a useful alternative to ELISA for diagnosing arboviral infections under field conditions.
免疫过氧化物酶单层分析法（IPMA）被用于检测针对六种虫媒病毒的抗体：黄病毒属的三种病毒（登革热2型、西尼罗河病毒和黄热病病毒）以及白蛉病毒属的三种病毒（裂谷热病毒、那不勒斯白蛉热病毒和西西里白蛉热病毒）。通过IPMA测定的同源超免疫小鼠腹水（HMAF）的抗体滴度比ELISA测定的低两到八倍。在用异源HMAF进行的检测中，ELISA中经常观察到的交叉反应，特别是在黄病毒属中，在所有IPMA滴定中均未出现。对于检测裂谷热病毒（n = 52）和西尼罗河病毒（n = 90）抗体的人血清样本，与ELISA相比，IPMA的灵敏度分别为96%和91%，IPMA的特异性为100%。此外，IPMA形式具有几个优点，使其成为在现场条件下诊断虫媒病毒感染时ELISA的有用替代方法。
Zotero 插件