Chen Z, Plagemann P G
Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455, USA.
J Virol Methods. 1997 May;65(2):227-36. doi: 10.1016/s0166-0934(97)02188-5.
It is known that lactate dehydrogenase-elevating virus (LDV) of mice is a common contaminant of transplantable tumors of both murine and human origin. It is imperative that tumors that are maintained by transplantation in mice are examined for LDV and freed of the virus, when present, before use in experimental studies, because an LDV infection of mice exerts considerable effects on lymphoid cell populations and cytokine production and other effects. Methods for LDV detection are described using a biological assay and reverse transcription (RT)-polymerase chain reaction (PCR) technology and their application is illustrated. A differential RT-PCR method that distinguishes between three quasispecies of LDV is also described and applied to an examination of LDVs isolated from a number of different tumors. Each of the LDV isolates was found to contain at least two different quasispecies, generally in different concentrations.
已知小鼠乳酸脱氢酶升高病毒（LDV）是源自鼠类和人类的可移植肿瘤的常见污染物。当用于实验研究时，必须对通过在小鼠体内移植来维持的肿瘤进行LDV检测，并在存在病毒时清除病毒，因为小鼠感染LDV会对淋巴细胞群体、细胞因子产生及其他方面产生相当大的影响。文中描述了使用生物测定法和逆转录（RT）-聚合酶链反应（PCR）技术进行LDV检测的方法，并举例说明了其应用。还描述了一种区分LDV三种准种的差异RT-PCR方法，并将其应用于对从多种不同肿瘤中分离出的LDV的检测。发现每个LDV分离株至少包含两种不同的准种，通常浓度不同。
