Peterson Norman C
MedImmune, One MedImmune Way, Gaithersburg, MD 20878, USA.
ILAR J. 2008;49(3):310-5. doi: 10.1093/ilar.49.3.310.
Many newly developed animal models involve the transfer of cells, serum, or other tissue-derived products into live rodents. These biologics can serve as repositories for adventitious rodent pathogens that, when used in animal studies, can alter research outcomes and result in endemic outbreaks. This review includes a description of some of the biologics that have inadvertently introduced infectious agents into in vivo studies and/or resulted in endemic outbreaks. I also discuss the points of potential exposure of specific biologics to adventitious rodent pathogens as well as the importance of acquiring a complete developmental and testing history of each biologic introduced into a barrier facility. There are descriptions of specific cases of mycoplasma and lactate dehydrogenase-elevating virus (LDHV), two of the most common organisms that contaminate cells and cell byproducts. The information in this article should help investigators and animal resource program personnel to perform an appropriate risk assessment of biologics before their use in in vivo studies that involve rodents.
许多新开发的动物模型涉及将细胞、血清或其他组织衍生产品转移到活体啮齿动物体内。这些生物制品可能成为偶发啮齿动物病原体的储存库，在用于动物研究时，可能会改变研究结果并导致地方性疫情爆发。本综述描述了一些在体内研究中意外引入感染因子和/或导致地方性疫情爆发的生物制品。我还讨论了特定生物制品接触偶发啮齿动物病原体的潜在暴露点，以及获取引入屏障设施的每种生物制品完整的研发和测试历史的重要性。文中描述了支原体和乳酸脱氢酶升高病毒（LDHV）的具体案例，这两种是污染细胞和细胞副产品最常见的生物体。本文中的信息应有助于研究人员和动物资源项目人员在将生物制品用于涉及啮齿动物的体内研究之前，对其进行适当的风险评估。
