Afeltra A, Ferri G M, Amoroso A, Leri O, Pellegrino C, Guido F, Garzia P, Paggi A
Department of Medicina Clinica, University of Rome, La Sapienza, Italy.
Endocr Res. 1997 Feb-May;23(1-2):81-94. doi: 10.1080/07435809709031844.
In a previous study we demonstrated a significant increase of CD5+ B subset in patients with Graves' disease (GD) compared with normal controls. The aim of this study was to compare the percentage of CD5+ B and CD5- B cells in GD with that in different forms of autoimmune and non immune-mediated thyroid diseases. Seventy-two patients were studied: 28 patients with GD, 20 with silent thyroiditis (ST), 12 with Hashimoto's disease (HD), and 12 subjects affected by hyperthyroidism due to toxic adenoma (TA). Eleven out of 28 patients with GD were also evaluated after six months of methimazole treatment. The study was performed by cytometric analysis. In GD the percentage and the absolute number of CD5+ B cells were significantly increased compared with normal controls (42.5 +/- 18.2% versus 19 +/- 6.3%, p < 0.0001; 142 +/- 153.3/cmm versus 46.9 +/- 22/cmm, p < 0.003, respectively. CD5+ B cells tended to normalise after six months of treatment. In ST the percentage of CD5+ B cells was increased (28.6 +/- 10.2%); conversely the absolute number was in the normal range. Patients affected by HD did not show any significant modification in B cells and their subsets in comparison with controls. In TA, CD5+ B were 7.6 /- 4.4% and 14.3 /- 10.9/cmm. Our results demonstrated a marked increase in both percentage and absolute number of CD5+ cells, only in active GD. The expansion of CD5+ B cells could play a role in the immune imbalance present in this disease.
在之前的一项研究中，我们证明，与正常对照组相比，格雷夫斯病（GD）患者的CD5+B细胞亚群显著增加。本研究的目的是比较GD患者与不同形式的自身免疫性和非免疫介导性甲状腺疾病患者的CD5+B细胞和CD5-B细胞百分比。我们对72例患者进行了研究：28例GD患者、20例寂静性甲状腺炎（ST）患者、12例桥本氏病（HD）患者以及12例因毒性腺瘤（TA）导致甲状腺功能亢进的患者。28例GD患者中有11例在接受甲巯咪唑治疗6个月后也进行了评估。研究通过细胞计数分析进行。与正常对照组相比，GD患者中CD5+B细胞的百分比和绝对数量均显著增加（分别为42.5±18.2%对19±6.3%，p<0.0001；142±153.3/立方毫米对46.9±22/立方毫米，p<0.003）。治疗6个月后，CD5+B细胞趋于正常化。ST患者中CD5+B细胞的百分比增加（28.6±10.2%）；相反，绝对数量在正常范围内。与对照组相比，HD患者的B细胞及其亚群未显示任何显著变化。在TA患者中，CD5+B细胞为7.6±4.4%和14.3±10.9/立方毫米。我们的结果表明，仅在活动期GD中，CD5+细胞的百分比和绝对数量均显著增加。CD5+B细胞的扩增可能在该疾病存在的免疫失衡中起作用。
