Ravichandran V, Roche P A
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
J Mol Neurosci. 1997 Apr;8(2):159-61. doi: 10.1007/BF02736780.
Syntaxins are transmembrane proteins that function in regulating transport vesicle docking and fusion with target membranes in neuronal and nonneuronal cells. Vesicle docking is thought to be regulated in part by the specific interactions of syntaxin with a vesicle-associated membrane protein termed synaptobrevin/VAMP. We have cloned a 1557-bp cDNA that encodes the human syntaxin 5 isoform, using a combination of PCR and colony-screening methods. The deduced 301 amino-acid sequence of human syntaxin 5 shares 96% identity with rat syntaxin 5. Like rat syntaxin 1A, human syntaxin 5 binds to synaptobrevin/VAMP in vitro. The identification of human syntaxin 5 as a synaptobrevin/VAMP-binding protein supports the hypothesis that syntaxin 5 regulates protein transport by binding to vesicle-associated membrane proteins.
Syntaxins是跨膜蛋白，在调节神经元和非神经元细胞中运输小泡与靶膜的对接和融合过程中发挥作用。小泡对接被认为部分受Syntaxin与一种称为突触小泡蛋白/囊泡相关膜蛋白（synaptobrevin/VAMP）的小泡相关膜蛋白的特异性相互作用调节。我们使用PCR和菌落筛选方法相结合，克隆了一个编码人类Syntaxin 5亚型的1557bp cDNA。推导的人类Syntaxin 5的301个氨基酸序列与大鼠Syntaxin 5有96%的同一性。与大鼠Syntaxin 1A一样，人类Syntaxin 5在体外与突触小泡蛋白/囊泡相关膜蛋白（synaptobrevin/VAMP）结合。将人类Syntaxin 5鉴定为突触小泡蛋白/囊泡相关膜蛋白（synaptobrevin/VAMP）结合蛋白，支持了Syntaxin 5通过与小泡相关膜蛋白结合来调节蛋白质运输的假说。
