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Cloning, sequencing and overexpression of the leucine dehydrogenase gene from Bacillus cereus.

作者信息

Stoyan T, Recktenwald A, Kula M R

机构信息

Institut für Enzymtechnologie, Heinrich-Heine Universität Düsseldorf in der KFA-Jülich, Germany.

出版信息

J Biotechnol. 1997 Apr 4;54(1):77-80. doi: 10.1016/s0168-1656(97)01670-2.

DOI:10.1016/s0168-1656(97)01670-2
PMID:9188201
Abstract

The L-leucine dehydrogenase gene from Bacillus cereus (DSM 626) was cloned from a partial genomic library and sequenced. The open reading frame has 1101 bp and codes for a protein of 39.9 kDa. The deduced amino acid sequence of the LeuDH from B. cereus shares 70-80% identity with LeuDH's from the thermophilic strains B. stearothermophilus and Thermoactinomyces intermedius. The active protein was overexpressed in Escherichia coli to yield approximately 30% of the total soluble protein.

摘要

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