Lundborg H, Hamberger A
Surgery. 1977 Nov;82(5):643-7.
In vivo and in vitro protein synthesis was measured in livers from rats 3 weeks after a portacaval shunt (PCS) operation. A marked reduction in protein synthesis was observed, amounting to a 66 and 47% of inhibition, respectively. Above-saturation levels of amino acids were used in both conditions. The level of free exogenous amino acid was elevated consistently in the PCS animals 1 hour after isotope injection. In support of the data on liver protein synthesis, the incorporated radioactivity in serum proteins was reduced markedly. Heart muscle protein synthesis was unchanged while brain protein synthesis was inhibited when assayed in vivo. Neuronal cells showed a more marked inhibition of protein synthesis than did glial cells.
在门腔静脉分流术（PCS）手术后3周的大鼠肝脏中测量了体内和体外蛋白质合成。观察到蛋白质合成显著减少，分别达到66%和47%的抑制率。在两种情况下均使用了高于饱和水平的氨基酸。在同位素注射后1小时，PCS动物体内游离外源性氨基酸水平持续升高。为支持肝脏蛋白质合成的数据，血清蛋白中掺入的放射性明显降低。心肌蛋白质合成未发生变化，而在体内检测时脑蛋白质合成受到抑制。神经元细胞比神经胶质细胞表现出更明显的蛋白质合成抑制。
