Helewski K, Konecki J
I Department of Histology and Embryology, Silesian Medical Academy, Zabrze-Rokitnica, Poland.
J Physiol Pharmacol. 1994 Dec;45(4):555-62.
Effects of portacaval shunts on the brain protein synthesis were studied in rats 7, 10 and 13 weeks after the surgery. We evaluated the incorporation of [3H]-glycine with the usage of liquid scintillation. In comparison to the controls, the labelled amino acid was incorporated less effectively in all the examined brain regions throughout the experimental period. The lowest values were observed 10 weeks after the portacaval shunt and the drop was most distinct in the cerebellum (47%). The least profound changes occurred within 7 weeks after the surgery and the results were statistically significant only in the frontal cortex and the brain stem. Thirteen weeks after the shunting the incorporation of [3H]-glycine was moderately decreased (the drop ranged between 12% and 18%) but all the differences were statistically significant. It seems that the inhibition of the brain protein synthesis may contribute to the pathogenesis of the portal-systemic encephalopathy.
在大鼠手术后7周、10周和13周，研究了门腔分流术对脑蛋白合成的影响。我们使用液体闪烁法评估了[3H]-甘氨酸的掺入情况。与对照组相比，在整个实验期间，标记氨基酸在所有检测的脑区中掺入效率较低。门腔分流术后10周观察到最低值，小脑下降最为明显（47%）。术后7周内变化最小，仅在额叶皮质和脑干中结果具有统计学意义。分流术后13周，[3H]-甘氨酸的掺入适度下降（下降幅度在12%至18%之间），但所有差异均具有统计学意义。似乎脑蛋白合成的抑制可能有助于门体性脑病的发病机制。
