Yoshiyama H, Imai S, Shimizu N, Takada K
Department of Virology, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.
J Virol. 1997 Jul;71(7):5688-91. doi: 10.1128/JVI.71.7.5688-5691.1997.
Recombinant Epstein-Barr virus (EBV) with a selectable marker successfully infected the human gastric carcinoma cell lines AGS, MKN28, and MKN74. Following incubation in selective media, drug-resistant cell clones were isolated and proved to be infected with EBV. All gastric carcinoma cell clones were positive for EBNA 1 but negative for EBNA 2. LMP 1 expression was negative in most clones, but there were a few exceptions. Gastric carcinoma cells were negative for the EBV receptor CD21, and infection was not inhibited by pretreatment of cells with the anti-CD21 monoclonal antibody OKB7. The results indicate that gastric carcinoma cells are susceptible to EBV infection and that infection is mediated via a new receptor different from CD21.
带有选择标记的重组爱泼斯坦-巴尔病毒（EBV）成功感染了人胃癌细胞系AGS、MKN28和MKN74。在选择性培养基中培养后，分离出耐药细胞克隆，并证明其感染了EBV。所有胃癌细胞克隆的EBNA 1呈阳性，但EBNA 2呈阴性。大多数克隆中LMP 1表达呈阴性，但有少数例外。胃癌细胞的EBV受体CD21呈阴性，用抗CD21单克隆抗体OKB7预处理细胞并不能抑制感染。结果表明，胃癌细胞易受EBV感染，且感染是通过不同于CD21的新受体介导的。
