[爱泼斯坦-巴尔病毒对人印戒细胞胃癌细胞系的非CD21依赖性感染]

[CD21-independent infection of a human signet ring cell gastric carcinoma cell line by Epstein-Barr virus].

作者信息

Luo Bing, Masanao Murakami, Makoto Fukuta, Kazuoshi Yanagihara, Takeshi Sairenji

机构信息

Department of Microbiology, Qingdao University Medical College, Qingdao 266021, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2004 Mar;18(1):59-61.

PMID:15340529

Abstract

OBJECTIVE

To understand Epstein-Barr virus (EBV) infection of gastric carcinoma cells.

METHODS

The authors tested the infection of a signet ring cell line HSC-39 derived from human gastric carcinoma with Akata and P3HR-1 strains of EBV. Akata and P3HR-1 infected of EBV cell clones were isolated by a limiting dilution method.

RESULTS

EBV-encoded small RNAs (EBERs) were expressed in the infected cells with each EBV strain by in situ hybridization. The EBV infected parental cells and most clones expressed EBNA1, but not EBNA2, latent membrane protein (LMP) 1 and LMP2A. Both EBV strains infected parental cells and clones presented type I latency. The uninfected HSC-39 cells were negative for CD21 expression; however, the Akata but not P3HR-1-infected clones were positive for CD21 expression at mRNA level.

CONCLUSION

These results demonstrated that EBV infecting HSC-39 by CD21-independent pathway. This study also defined a signet ring cell line as a new target for EBV.

摘要

目的

了解胃癌细胞的爱泼斯坦-巴尔病毒（EBV）感染情况。

方法

作者用EBV的Akata和P3HR-1毒株检测源自人胃癌的印戒细胞系HSC-39的感染情况。通过有限稀释法分离出Akata和P3HR-1感染的EBV细胞克隆。

结果

通过原位杂交，EBV编码的小RNA（EBERs）在每种EBV毒株感染的细胞中表达。EBV感染的亲代细胞和大多数克隆表达EBNA1，但不表达EBNA2、潜伏膜蛋白（LMP）1和LMP2A。两种EBV毒株感染的亲代细胞和克隆均呈现I型潜伏状态。未感染的HSC-39细胞CD21表达呈阴性；然而，Akata感染的克隆在mRNA水平上CD21表达呈阳性，而P3HR-1感染的克隆则为阴性。