哮喘患者的血清嗜酸性粒细胞过氧化物酶（EPO）水平。

Serum eosinophil peroxidase (EPO) levels in asthmatic patients.

作者信息

Sanz M L, Parra A, Prieto I, Diéguez I, Oehling A K

机构信息

Department of Allergology and Clinical Immunology, University Clinic, Faculty of Medicine, University of Navarra, Pamplona, Spain.

出版信息

Allergy. 1997 Apr;52(4):417-22. doi: 10.1111/j.1398-9995.1997.tb01021.x.

DOI:10.1111/j.1398-9995.1997.tb01021.x

PMID:9188923

Abstract

Eosinophil granular proteins are a useful eosinophilic activation marker in asthmatic patients. In this study, the eosinophil peroxidase (EPO) levels were assessed in different stages of bronchial asthma, in 123 patients suffering from asthma, classified as mild (n =49), moderate (n = 49), and severe (n = 25), according to the International Consensus Report of Diagnosis and Treatment of Asthma, as well as in 27 healthy controls, with the aim of evaluating the importance of this protein as a severity marker in bronchial asthma, and its possible correlation with parameters such as anamnesis, respiratory function tests, and peripheral blood eosinophil count, and also with some allergologic diagnostic tests, both in vivo and in vitro. The geometric mean serum level of EPO was 9.3 +/- 11.3 ng/ml (median +/- SD) in controls, 28 +/- 37.8 ng/ml in the asthmatic patients. Depending on the asthma severity, the EPO levels were 25 +/- 30.5; 29 +/- 37.1, and 41 +/- 47.3 ng/ml in mild, moderate, and severe asthmatics, respectively, being the significant differences between the group of patients with mild and severe asthma (P < 0.001). The number of eosinophils (eos) in peripheral blood was 157 +/- 20 eos/mm3 in the controls, 334/35 eos/mm3 in mild asthmatics, 510 +/- 87 eos/mm3 in moderate asthmatics, and 658 +/- 72 eos/mm3 in severe asthmatics, with significant differences between all groups (from P < 0.05 to P < 0.001). Both the serum levels of EPO and the number of eosinophils were greater in patients with active asthma patients with inactive asthma (P < 0.001). Significant negative correlations (P < 0.001) were found between serum levels of EPO and FEV1 (rs = 0.30), MEF25-75 (rs = -0.33) and MEF50 (rs = -0.34), and a good positive correlation (rs = 0.80, P < 0.001) was found between EPO levels and the number of eosinophils in peripheral blood. We also found a significant positive correlation between eosinophil number and clinical score (rs = 0.54, P < 0.001) and between EPO levels and the mentioned score (rs = 0.46, P < 0.001).

摘要

嗜酸性粒细胞颗粒蛋白是哮喘患者有用的嗜酸性粒细胞活化标志物。在本研究中，根据哮喘诊断和治疗国际共识报告，对123例哮喘患者（分为轻度组n = 49、中度组n = 49和重度组n = 25）以及27名健康对照者的嗜酸性粒细胞过氧化物酶（EPO）水平进行了评估，目的是评估该蛋白作为支气管哮喘严重程度标志物的重要性，及其与诸如既往史、呼吸功能测试、外周血嗜酸性粒细胞计数等参数，以及一些体内和体外变应性诊断测试之间的可能相关性。对照组EPO的几何平均血清水平为9.3±11.3 ng/ml（中位数±标准差），哮喘患者为28±37.8 ng/ml。根据哮喘严重程度，轻度、中度和重度哮喘患者的EPO水平分别为25±30.5、29±37.1和41±47.3 ng/ml，轻度和重度哮喘患者组之间存在显著差异（P < 0.001）。对照组外周血嗜酸性粒细胞数量为157±20个/立方毫米，轻度哮喘患者为334/35个/立方毫米，中度哮喘患者为510±87个/立方毫米，重度哮喘患者为658±72个/立方毫米，所有组之间均存在显著差异（P值从< 0.05至< 0.001）。活动期哮喘患者的EPO血清水平和嗜酸性粒细胞数量均高于非活动期哮喘患者（P < 0.001）。发现EPO血清水平与FEV1（rs = 0.30）、MEF25 - 75（rs = - 0.33）和MEF50（rs = - 0.34）之间存在显著负相关（P < 0.001），EPO水平与外周血嗜酸性粒细胞数量之间存在良好的正相关（rs = 0.80，P < 0.001）。我们还发现嗜酸性粒细胞数量与临床评分之间存在显著正相关（rs = 0.54，P < 0.001），EPO水平与上述评分之间也存在显著正相关（rs = 0.46，P < 0.001）。