Dvorak A M
Department of Pathology, Beth Israel Hospital, Massachusetts 02215, USA.
Allergy. 1997;52(34 Suppl):14-24. doi: 10.1111/j.1398-9995.1997.tb04807.x.
Subcellular sites of histamine in purified human lung mast cells and human peripheral blood basophils were determined using a newly developed enzyme-gold affinity method for ultrastructural analysis. The evolving morphologies of regulated secretion and recovery from regulated secretion in each cell showed that granule stores of histamine were released at early times after stimulation and re-accumulated at later times post-stimulus. Renewal of histamine granule stores occurred by multiple mechanisms including synthesis and conservation of granule materials and membranes.
利用一种新开发的用于超微结构分析的酶-金亲和方法，确定了纯化的人肺肥大细胞和人外周血嗜碱性粒细胞中组胺的亚细胞定位。每个细胞中受调节分泌的演变形态以及从受调节分泌中恢复的形态表明，组胺颗粒储存库在刺激后的早期释放，并在刺激后的后期重新积累。组胺颗粒储存库的更新通过多种机制发生，包括颗粒物质和膜的合成与保存。
