Dvorak A M, Morgan E S
Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA.
Histochem J. 1998 Aug;30(8):603-8. doi: 10.1023/a:1003274915675.
Basophilic leucocytes are metachromatic granule-containing secretory granulocytes that contain a mixture of granular proteoglycans devoid of heparin. In guinea pigs, isolated basophilic leucocyte granules primarily contain chondroitin sulphate. We have recently demonstrated that an enzyme-affinity-gold technique to image RNA, using the reagent RNase gold, also binds specifically to heparin in human mast cell granules. Such binding is based on the known property of heparin as a competitive inhibitor of RNase. Using similar methods, we show here that RNase-gold binds to the chondroitin sulphate in the secretory granules of guinea pig basophils, thus broadening the applicability of this post-embedding affinity-gold method to studies that require imaging of chondroitin sulphate in routinely prepared electron microscopical samples.
嗜碱性白细胞是含有异染颗粒的分泌性粒细胞，其含有不含肝素的颗粒蛋白聚糖混合物。在豚鼠中，分离出的嗜碱性白细胞颗粒主要含有硫酸软骨素。我们最近证明，一种使用试剂核糖核酸酶金对RNA进行成像的酶亲和金技术，也能特异性地结合人肥大细胞颗粒中的肝素。这种结合基于肝素作为核糖核酸酶竞争性抑制剂的已知特性。使用类似方法，我们在此表明核糖核酸酶金能结合豚鼠嗜碱性粒细胞分泌颗粒中的硫酸软骨素，从而扩大了这种包埋后亲和金方法在常规制备的电子显微镜样品中对硫酸软骨素成像研究的适用性。
