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胰岛素免疫分析中的抗胰岛素抗体：一个仍可能存在的陷阱。

Anti-insulin antibodies in insulin immunometric assays: a still possible pitfall.

作者信息

Sapin R

机构信息

Laboratoire Universitaire de Biophysique, CNRS URA 1173, Faculté de Médecine, Strasbourg, France.

出版信息

Eur J Clin Chem Clin Biochem. 1997 May;35(5):365-7. doi: 10.1515/cclm.1997.35.5.365.

DOI:10.1515/cclm.1997.35.5.365
PMID:9189740
Abstract

Insulin was assayed directly using radioimmunoassay and immunometric assay in 31 sera containing anti-insulin antibodies. Anti-insulin antibodies were determined by radio-binding-assay. Insulin measurements were compared with those of free (unbound to antibodies, polyethylene glycol precipitated) insulin measurements. Compared with free insulin concentrations, radioimmunoassay and immunometric assay yielded falsely increased insulin results. The degree of overestimation by radioimmunoassay and by immunometric assay correlated with the anti-insulin antibody value. Anti-insulin antibodies still remain a possible pitfall in the insulin-specific immunometric assays which are now being widely used.

摘要

采用放射免疫分析法和免疫比浊法对31份含有抗胰岛素抗体的血清进行胰岛素直接检测。通过放射结合分析法测定抗胰岛素抗体。将胰岛素检测结果与游离（未与抗体结合、经聚乙二醇沉淀）胰岛素的检测结果进行比较。与游离胰岛素浓度相比，放射免疫分析法和免疫比浊法得出的胰岛素结果存在假性升高。放射免疫分析法和免疫比浊法的高估程度与抗胰岛素抗体值相关。抗胰岛素抗体仍是目前广泛使用的胰岛素特异性免疫比浊法中一个可能存在的问题。

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