Kuhr L P, Baum H, Schweigert R, Hafner G, Prellwitz W, Neumeier D
Institut für Klinische Chemie und Pathobiochemie, Klinikum rechts der Isar der Technischen Universität München, Germany.
Eur J Clin Chem Clin Biochem. 1997 May;35(5):399-404.
We evaluated a rapid, quantitative immunoassay for the detection of cardiac troponin I. Coefficient of variation is between 1.29 and 13.63% for intra-assay and between 3.88 and 10.15% for inter-assay imprecision. Linearity is given up to 35 micrograms/l. Possible interfering substances (haemoglobin, bilirubin, triacylglycerol and rheuma factors) do not disturb the assay. The analyte is stable under normal storage conditions (+20 degrees C/48 h and +4 degrees C/l week) with decrease up to 30% after 3 months at -20 degrees C. Reference value for apparently healthy individuals is < 0.1 microgram/l. In plasma cardiac troponin I is measured up to 30% depressed compared to serum. Comparison with another cardiac troponin I assay (y = 0.92x + 2.42, r = 0.940) and cardiac troponin T is good with y = 6.61x - 1.94, r = 0.91 for the first generation cardiac troponin T assay and y = 5.59x - 0.68, r = 0.87 for the second generation cardiac troponin T assay. In summary, the evaluated assay is fast, easy to perform, and can be used not only in a specialized laboratory, but is also suitable for emergency laboratory or smaller laboratory units.
我们评估了一种用于检测心肌肌钙蛋白I的快速定量免疫测定法。批内变异系数在1.29%至13.63%之间，批间不精密度在3.88%至10.15%之间。线性范围可达35微克/升。可能的干扰物质（血红蛋白、胆红素、三酰甘油和类风湿因子）不会干扰该测定。在正常储存条件下（+20℃/48小时和+4℃/1周）分析物稳定，在-20℃下3个月后下降高达30%。明显健康个体的参考值<0.1微克/升。与血清相比，血浆中心肌肌钙蛋白I的测量值低30%。与另一种心肌肌钙蛋白I测定法比较（y = 0.92x + 2.42，r = 0.940），与第一代心肌肌钙蛋白T测定法比较（y = 6.61x - 1.94，r = 0.91）以及与第二代心肌肌钙蛋白T测定法比较（y = 5.59x - 0.68，r = 0.87）结果良好。总之，所评估的测定法快速、易于操作，不仅可用于专业实验室，也适用于急诊实验室或较小的实验室单元。
