Satyaswaroop P G, Lopez de la Osa E, Gurpide E
Steroids. 1977 Jul;30(1):139-45. doi: 10.1016/0039-128x(77)90144-1.
High Pressure liquid chromatographic procedures for the resolution of mixtures of C18 and C19 steroids of biologic importance are described. On-line monitoring of the absorbance and refractive index of the eluate was found to be suitable for the quantitative measurement of the amounts of eluted steroids. Using a high sensitivity absorbance detector, reliable measurements of as little as 5 ng delta(3)-3 keto steroids were possible.
描述了用于分离具有生物学重要性的C18和C19类固醇混合物的高压液相色谱方法。发现对洗脱液的吸光度和折射率进行在线监测适用于定量测定洗脱类固醇的量。使用高灵敏度吸光检测器，可以可靠地测量低至5 ng的δ(3)-3-酮类固醇。
