Roos R W
J Assoc Off Anal Chem. 1980 Jan;63(1):80-7.
The use of high pressure liquid chromatography for separating mixtures of various estrogens is described. Separations of as many as 9 estrogens were studied using normal and reverse phase partition systems; the reverse phase system was the most useful. The estrogens were detected at 254 nm, a wavelength that favors their detection at low concentrations. The results of the separation studies were applied successfully to the separation and identification of estrogens in aqueous suspensions of estrogenic substances and to conjugated estrogens in tablets, following hydrolysis of the conjugates. Most estrogen peaks were identified using chromatographic mobilities; in some cases chemical reaction with the estrogen was used to form derivatives to aid identification. In one instance, the ultraviolet-absorbing properties of the estrogen were compared with literature data in order to assign an identity.
本文描述了使用高压液相色谱法分离各种雌激素混合物的方法。使用正相和反相分配系统研究了多达9种雌激素的分离；反相系统最为有用。雌激素在254nm处检测，该波长有利于低浓度下的检测。分离研究结果成功应用于雌激素物质水悬浮液中雌激素的分离和鉴定，以及片剂中结合雌激素在结合物水解后的分离和鉴定。大多数雌激素峰通过色谱迁移率进行鉴定；在某些情况下，利用与雌激素的化学反应形成衍生物以辅助鉴定。在一个实例中，将雌激素的紫外吸收特性与文献数据进行比较以确定其身份。
