琥珀酸脱氢酶基因（sdh）的突变会改变鼠伤寒沙门氏菌对硫胺素的需求。
Mutations in sdh (succinate dehydrogenase genes) alter the thiamine requirement of Salmonella typhimurium.
作者信息
Enos-Berlage J L, Downs D M
机构信息
Department of Bacteriology, University of Wisconsin-Madison, 53706, USA.
出版信息
J Bacteriol. 1997 Jun;179(12):3989-96. doi: 10.1128/jb.179.12.3989-3996.1997.
Abstract
Mutants lacking the first enzyme in de novo purine synthesis (PurF) can synthesize thiamine if increased levels of pantothenate are present in the culture medium (J. L. Enos-Berlage and D. M. Downs, J. Bacteriol. 178:1476-1479, 1996). Derivatives of purF mutants that no longer required pantothenate for thiamine-independent growth were isolated. Analysis of these mutants demonstrated that they were defective in succinate dehydrogenase (Sdh), an enzyme of the tricarboxylic acid cycle. Results of phenotypic analyses suggested that a defect in Sdh decreased the thiamine requirement of Salmonella typhimurium. This reduced requirement correlated with levels of succinyl-coenzyme A (succinyl-CoA), which is synthesized in a thiamine pyrophosphate-dependent reaction. The effect of succinyl-CoA on thiamine metabolism was distinct from the role of pantothenate in thiamine synthesis.
摘要
如果培养基中泛酸盐水平升高，缺乏从头嘌呤合成途径中第一种酶（PurF）的突变体能够合成硫胺素（J. L. 伊诺斯 - 伯拉格和D. M. 唐斯，《细菌学杂志》178:1476 - 1479，1996年）。分离得到了不再需要泛酸盐就能在不依赖硫胺素的情况下生长的purF突变体衍生物。对这些突变体的分析表明，它们在三羧酸循环的一种酶——琥珀酸脱氢酶（Sdh）中存在缺陷。表型分析结果表明，Sdh缺陷降低了鼠伤寒沙门氏菌对硫胺素的需求。这种需求的降低与琥珀酰辅酶A（琥珀酰 - CoA）的水平相关，琥珀酰辅酶A是在硫胺素焦磷酸依赖反应中合成的。琥珀酰辅酶A对硫胺素代谢的影响与泛酸盐在硫胺素合成中的作用不同。
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