多个转录元件控制大肠杆菌btuB基因的表达。
Multiple transcribed elements control expression of the Escherichia coli btuB gene.
作者信息
Franklund C V, Kadner R J
机构信息
Department of Microbiology, University of Virginia School of Medicine, Charlottesville 22908, USA.
出版信息
J Bacteriol. 1997 Jun;179(12):4039-42. doi: 10.1128/jb.179.12.4039-4042.1997.
Abstract
Repression by vitamin B12 of the cobalamin transport protein BtuB in the outer membrane of Escherichia coli operates at both the transcriptional and translational levels and is controlled by transcribed sequences within the leader and proximal portion of the btuB coding sequence. The effects of deletions from either end of this region on repression and expression were determined with lac fusions. An element at the 5' end of the transcript and the putative attenuator within the coding sequence were required for transcriptional repression. The presence of either element caused a marked reduction in btuB-lacZ expression which was reversed by the presence of a conserved sequence element in the leader, suggesting the importance of long-range interactions in the btuB leader for expression and regulation.
摘要
维生素B12对大肠杆菌外膜中钴胺素转运蛋白BtuB的抑制作用在转录和翻译水平上均有发生，且受btuB编码序列前导区和近端部分内转录序列的控制。利用lac融合技术确定了该区域两端缺失对抑制作用和表达的影响。转录抑制需要转录本5'端的一个元件和编码序列内的假定衰减子。这两个元件中的任何一个的存在都会导致btuB - lacZ表达显著降低，而前导区中一个保守序列元件的存在可逆转这种降低，这表明btuB前导区中远距离相互作用对表达和调控的重要性。
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本文引用的文献
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Vitamin B12 repression of the btuB gene in Salmonella typhimurium is mediated via a translational control which requires leader and coding sequences.鼠伤寒沙门氏菌中维生素B12对btuB基因的抑制作用是通过一种翻译控制介导的，这种翻译控制需要前导序列和编码序列。
Mol Microbiol. 1997 Jan;23(1):35-42. doi: 10.1046/j.1365-2958.1997.1761543.x.
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Cobalamin (coenzyme B12): synthesis and biological significance.钴胺素（辅酶B12）：合成与生物学意义
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Regulation of expression of the gene for vitamin B12 receptor cloned on a multicopy plasmid in Escherichia coli.在大肠杆菌中，多拷贝质粒上克隆的维生素B12受体基因表达的调控。
Mol Gen Genet. 1986 Nov;205(2):358-65. doi: 10.1007/BF00430451.
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Altered cobalamin metabolism in Escherichia coli btuR mutants affects btuB gene regulation.大肠杆菌btuR突变体中钴胺素代谢的改变影响btuB基因调控。
J Bacteriol. 1989 Jan;171(1):154-61. doi: 10.1128/jb.171.1.154-161.1989.
10
Transcribed sequences of the Escherichia coli btuB gene control its expression and regulation by vitamin B12.大肠杆菌btuB基因的转录序列通过维生素B12控制其表达和调控。
Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1479-83. doi: 10.1073/pnas.88.4.1479.
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