鼠伤寒沙门氏菌中钴胺素生物合成操纵子的调控
Regulation of cobalamin biosynthetic operons in Salmonella typhimurium.
作者信息
Escalante-Semerena J C, Roth J R
出版信息
J Bacteriol. 1987 May;169(5):2251-8. doi: 10.1128/jb.169.5.2251-2258.1987.
Abstract
Transcription of cobalamin (cob) biosynthetic genes in Salmonella typhimurium is repressed by cobalamin and by molecular oxygen. These genes seem to be subject to catabolite repression, and they are maximally expressed under conditions of anaerobic respiration of glycerol-fumarate. A 215-fold increase in the expression of cob genes occurs when S. typhimurium shifts from aerobic growth on glucose to anaerobic respiration of glycerol-fumarate under strictly anoxic growth conditions. Exogenous cyclic AMP substantially stimulates the transcription of cob-lac fusions during aerobic growth. However, cyclic AMP is not absolutely required for the expression of the pathway, nor does it mediate the aerobic control. Cobalamin biosynthesis is not seen under aerobic growth conditions, even when transcription is stimulated by the addition of cyclic AMP. Hence, additional control mechanisms triggered by the presence of molecular oxygen must operate independently from transcription effects on the cob operons.
摘要
鼠伤寒沙门氏菌中钴胺素（cob）生物合成基因的转录受到钴胺素和分子氧的抑制。这些基因似乎受到分解代谢物阻遏，并且在甘油-富马酸酯的厌氧呼吸条件下表达量最高。当鼠伤寒沙门氏菌在严格缺氧的生长条件下从以葡萄糖进行有氧生长转变为以甘油-富马酸酯进行厌氧呼吸时，cob基因的表达增加了215倍。外源性环腺苷酸在有氧生长期间显著刺激cob-lac融合体的转录。然而，环腺苷酸对于该途径的表达并非绝对必需，它也不介导有氧调控。即使通过添加环腺苷酸刺激转录，在有氧生长条件下也看不到钴胺素的生物合成。因此，由分子氧的存在触发的额外调控机制必须独立于对cob操纵子的转录效应发挥作用。
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本文引用的文献
1
Regulation of methionine synthesis in Escherichia coli: Effect of metJ gene product and S-adenosylmethionine on the expression of the metF gene.大肠杆菌甲硫氨酸合成的调控：metJ 基因产物和 S-腺苷甲硫氨酸对 metF 基因表达的影响。
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3601-5. doi: 10.1073/pnas.82.11.3601.
2
Ring contraction of hydroporphinoid to corrinoid complexes.氢卟啉环收缩为钴胺素类复合物。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):16-9. doi: 10.1073/pnas.78.1.16.
3
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
4
Why a co-substrate is required for anaerobic growth of Escherichia coli on citrate.为什么在柠檬酸盐上大肠杆菌厌氧生长需要共底物。
J Gen Microbiol. 1980 Jul;119(1):63-70. doi: 10.1099/00221287-119-1-63.
5
Biosynthesis of vitamin B12: mode of incorporation of factor III into cobyrinic acid.维生素B12的生物合成：因子III掺入钴胺酸的方式。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):9-10. doi: 10.1073/pnas.78.1.9.
6
Biosynthesis of vitamin B12: identity of fragment extruded during ring contraction to the corrin macrocycle.维生素B12的生物合成：在向咕啉大环收缩过程中挤出片段的鉴定。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):13-5. doi: 10.1073/pnas.78.1.13.
7
Biosynthesis of vitamin B12: nature of the volatile fragment generated during formation of the corrin ring system.维生素B12的生物合成：在咕啉环系统形成过程中产生的挥发性片段的性质。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):11-2. doi: 10.1073/pnas.78.1.11.
8
Biosynthesis of vitamin B12. Different pathways in some aerobic and anaerobic microorganisms.维生素B12的生物合成。一些需氧和厌氧微生物中的不同途径。
Arch Microbiol. 1982 Aug;132(2):155-8. doi: 10.1007/BF00508722.
9
Biosynthesis of vitamin B12. Some properties of the 5,6-dimethylbenzimidazole-forming system of Propionibacterium freudenreichii and Propionibacterium shermanii.维生素B12的生物合成。费氏丙酸杆菌和谢氏丙酸杆菌中5,6-二甲基苯并咪唑形成系统的一些特性。
Eur J Biochem. 1980 Apr;105(3):587-92. doi: 10.1111/j.1432-1033.1980.tb04536.x.
10
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J Bacteriol. 1984 Jul;159(1):206-13. doi: 10.1128/jb.159.1.206-213.1984.
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