Estes M K, Ball J M, Crawford S E, O'Neal C, Opekun A A, Graham D Y, Conner M E
Division of Molecular Virology, Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.
Adv Exp Med Biol. 1997;412:387-95. doi: 10.1007/978-1-4899-1828-4_61.
Viruses which infect the gastrointestinal tract are well suited for examining the immune response(s) to oral delivery of antigen and exploring the advantages and pitfalls of oral vaccines. We have used recombinant DNA techniques to produce nonreplicating self-assembled virus-like particles (VLPs) from two gastrointestinal viruses, rotavirus and Norwalk virus. Both of these viruses normally cause acute gastroenteritis in man or animals. The VLPs are morphologically and antigenically similar to the native virus and quite stable, features which are advantageous for their use as subunit vaccines. In addition, these VLPs could be useful as carriers of foreign epitopes from heterologous pathogens or of drugs which need to be delivered to the gastrointestinal track. This paper briefly reviews the properties of these VLPs made in insect cells and data showing their potential as subunit vaccines for parenteral or oral delivery.
感染胃肠道的病毒非常适合用于研究口服抗原后的免疫反应，以及探索口服疫苗的优缺点。我们利用重组DNA技术从两种胃肠道病毒——轮状病毒和诺如病毒中制备了非复制性自组装病毒样颗粒（VLP）。这两种病毒通常会在人类或动物中引发急性肠胃炎。这些VLP在形态和抗原性上与天然病毒相似，且相当稳定，这些特性使其有利于用作亚单位疫苗。此外，这些VLP还可作为来自异源病原体的外源表位或需要递送至胃肠道的药物的载体。本文简要综述了在昆虫细胞中制备的这些VLP的特性，以及表明它们作为用于肠胃外或口服递送的亚单位疫苗的潜力的数据。
