口腔鳞状细胞癌活检中的p53。一项与恶性肿瘤分级系统的比较研究。

p53 In biopsies of oral squamous cell carcinoma. A comparative study with a malignancy grading system.

作者信息

De Araújo V C, Loyola A M, Pinto Júnior D D, Borra R C, De Araújo N S

机构信息

Disciplina de Patalogia Bucal, Faculdade de Odontologia, Universidade do São Paulo, Brazil.

出版信息

Oral Oncol. 1997 Jan;33(1):5-9. doi: 10.1016/s0964-1955(96)00055-3.

DOI:10.1016/s0964-1955(96)00055-3

PMID:9192545

Abstract

The relationship between the histological grading of malignancy and p53 protein expression was studied in 40 biopsies of oral squamous cell carcinomas. An immunohistochemical analysis was carried out using the streptavidin method preceded by a treatment with citric acid in a microwave oven. All cases were classified according to the histological malignancy grading system proposed by Anneroth et al. (Scandinavian Journal of Dental Research 1987, 95, 229-249). The expression of p53 was found in 62.5% of the carcinomas studied. Positivity of p53 staining showed a correlation with the histological grade of malignancy and with the degree of keratinisation, nuclear polymorphism and number of mitoses.

摘要

对40例口腔鳞状细胞癌活检标本研究了恶性组织学分级与p53蛋白表达之间的关系。采用链霉抗生物素蛋白法进行免疫组织化学分析，分析前用柠檬酸在微波炉中处理。所有病例均根据Anneroth等人提出的组织学恶性分级系统进行分类（《斯堪的纳维亚牙科研究杂志》1987年，95卷，229 - 249页）。在所研究的癌组织中，62.5%发现有p53表达。p53染色阳性与恶性组织学分级以及角化程度、核多形性和有丝分裂数相关。