McKiernan J M, Buttyan R, Bander N H, Stifelman M D, Katz A E, Chen M W, Olsson C A, Sawczuk I S
Department of Urology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Cancer Res. 1997 Jun 15;57(12):2362-5.
MN is a novel cell surface antigen originally detected in human HeLa cells. Although it is also expressed in normal gastric mucosa, this antigen was previously found to be expressed in cells with a malignant phenotype in certain tissues of the female genital tract (cervix and ovary). Using an oligonucleotide primer set specific for MN-complimentary DNA, we performed reverse transcription-PCR assays on RNAs extracted from human cell lines and tissues to evaluate whether this marker might be expressed at other sites. RNA libraries extracted from normal human heart, lung, kidney, prostate, peripheral blood, brain, placenta, and muscle were negative for MN expression. RNAs extracted from liver and pancreatic tissue were positive for MN expression. Three of six renal cancer cell lines tested revealed MN expression. In addition, 12 of 17 samples of human renal cell carcinoma tissue tested positive for MN, all 12 of which were clear cell adenocarcinomas. This survey identified a unique association of MN expression with renal cell cancers, especially those of the clear cell variety, suggesting that MN is a potential marker for the diagnosis, staging, and therapeutic monitoring of renal cell carcinoma in humans.
MN是一种最初在人宫颈癌细胞中检测到的新型细胞表面抗原。尽管它也在正常胃黏膜中表达，但此前发现该抗原在女性生殖道某些组织（宫颈和卵巢）中具有恶性表型的细胞中表达。我们使用针对MN互补DNA的寡核苷酸引物组，对从人细胞系和组织中提取的RNA进行逆转录-聚合酶链反应（RT-PCR）分析，以评估该标志物是否可能在其他部位表达。从正常人心脏、肺、肾、前列腺、外周血、脑、胎盘和肌肉中提取的RNA文库中，MN表达呈阴性。从肝脏和胰腺组织中提取的RNA，MN表达呈阳性。所检测的六种肾癌细胞系中有三种显示出MN表达。此外，在检测的17份人肾细胞癌组织样本中，有12份MN呈阳性，其中所有12份均为透明细胞腺癌。这项调查确定了MN表达与肾细胞癌，尤其是透明细胞型肾细胞癌之间的独特关联，表明MN是人类肾细胞癌诊断、分期和治疗监测的潜在标志物。
