Mukherji S K, Schiro S, Castillo M, Kwock L, Muller K E, Blackstock W
Department of Radiology, University of North Carolina School of Medicine, Chapel Hill 27599, USA.
AJNR Am J Neuroradiol. 1997 Jun-Jul;18(6):1057-72.
To determine the ability of in vitro one-dimensional and two-dimensional proton MR spectroscopy to help differentiate squamous cell carcinoma of the extracranial head and neck from normal tissues and to correlate the in vitro observations with clinical studies.
In vitro 1-D and 2-D correlated proton MR spectroscopy (11 T) was performed in tissue specimens of squamous cell carcinoma of the head and neck (n = 19), in normal tissue (n = 13), in metastatic cervical lymph nodes (n = 3), and in a squamous cell carcinoma cell line. In vivo 1-D proton MR spectroscopy (1.5 T) was performed in patients with squamous cell carcinoma (n = 7) and in healthy volunteers (n = 7). The ratio of the areas under the choline (Cho) and creatine (Cr) resonances were calculated for 1-D proton MR spectra for the in vitro tissue studies and correlated with the in vivo studies. Data from in vitro 2-D correlated spectroscopy were analyzed for differences in the presence or absence of various metabolites in samples of tumor and normal tissue. Statistical analysis consisted of 2 x 2 factorial repeated measures analysis of variance (ANOVA), discriminate analysis, and chi2 test.
The mean in vitro 1-D proton MR spectroscopic Cho/Cr ratio was significantly higher in tumor than in normal tissue. The difference between the mean ratios appeared to increase with increasing echo time. All in vivo tumor Cho/Cr ratios were greater than the calculated mean in vitro tumor ratio, whereas six of the seven volunteers had no detectable Cho and Cr resonances. Two-dimensional correlated MR spectroscopic data revealed that a variety of amino acids have a significantly greater likelihood of being detected in tumor than in normal tissues.
One-dimensional and 2-D proton MR spectroscopy can help differentiate primary squamous cell carcinoma and nodal metastases containing squamous cell carcinoma from normal tissue both in vitro and in vivo. In addition, 2-D spectroscopy can help identify the presence of certain amino acids in squamous cell carcinoma that are not detected in normal tissue.
确定体外一维和二维质子磁共振波谱技术帮助鉴别颅外头颈部鳞状细胞癌与正常组织的能力，并将体外观察结果与临床研究相关联。
对头颈部鳞状细胞癌组织标本（n = 19）、正常组织（n = 13）、转移性颈部淋巴结（n = 3）以及鳞状细胞癌细胞系进行体外一维和二维相关质子磁共振波谱（11 T）检查。对鳞状细胞癌患者（n = 7）和健康志愿者（n = 7）进行体内一维质子磁共振波谱（1.5 T）检查。计算体外组织研究中一维质子磁共振波谱中胆碱（Cho）和肌酸（Cr）共振峰下面积的比值，并与体内研究结果相关联。分析体外二维相关波谱数据，以确定肿瘤和正常组织样本中各种代谢物的有无差异。统计分析包括2×2析因重复测量方差分析（ANOVA）、判别分析和卡方检验。
肿瘤组织中体外一维质子磁共振波谱的平均Cho/Cr比值显著高于正常组织。平均比值之间的差异似乎随着回波时间的增加而增大。所有体内肿瘤的Cho/Cr比值均高于计算出的体外肿瘤平均比值，而7名志愿者中有6名未检测到Cho和Cr共振峰。二维相关磁共振波谱数据显示，与正常组织相比，肿瘤中多种氨基酸被检测到的可能性显著更高。
一维和二维质子磁共振波谱技术能够在体外和体内帮助鉴别原发性鳞状细胞癌以及含有鳞状细胞癌的淋巴结转移灶与正常组织。此外，二维波谱技术能够帮助识别鳞状细胞癌中正常组织未检测到的某些氨基酸的存在。
