[Protein metabolism in human embryo skin-muscle tissue cells infected with oncornavirus D from J-96 cells].

Structural polypeptides of oncornavirus D produced by J-96 cells and the effect of oncornavirus infection on protein metabolism of human embryo skin-muscle tissue cells (HESM) were studied. Electrophoresis showed the number of polypeptides detectable in a preparation of virus produced by infected HESM cells to depend on the number of centrifugations in sucrose gradient in the process of virus purification. A preparation of a singly purified virus was found to contain 13 polypeptide components: p115, p110, p100, p87, p78, p69, p57, p45, p36, p27, p15, p12, and p10. In oncornavirus D from J-96 cells purified three times in sucrose gradient 2 major polypeptides were found with molecular weights 69,000 and 27,000 daltons which had about 80% of the total radioactivity, and minor polypeptides p15, p12, and p10. Comparison of electrophoregrams of proteins of HEMS cells infected with oncornavirus D and uninfected cells showed oncornavirus infection to be accompanied by an increased synthesis of some polypeptides (p115, p87, p69) and a decreased amount of others (p78, p36, p17-p12). Besides, in infected HESM cells two proteins were found the molecular weights of which coincided with those of oncornavirus polypeptides p69 and p27. The p69 protein component was detected in the membrane fraction of a continuous line of transformed J-96 cells labeled with 3H-glucosamine indicating its complex glycoprotein nature. The results suggest that the observed changes in metabolism of cell proteins in oncornavirus infection of HESM cells do not result in transformation of these cells or are insufficient for its occurrence.