Crossley A, Calvert J E, Taylor P R, Dickinson A M
Department of Haematology, Medical School, University of Newcastle upon Tyne, UK.
Transfus Med. 1997 Jun;7(2):127-34. doi: 10.1046/j.1365-3148.1997.d01-15.x.
In this study we have compared two assays for the detection of autoantibodies GpIIb/IIIa, platelet bound and in serum, in immune thrombocytopenic purpura (ITP). Both assays were found to have a similar sensitivity, but the monoclonal antibody immobilization of platelet antigen (MAIPA) assay was more reproducible than the immunobead assay. The MAIPA and immunobead assay demonstrated an 81% concordance of results for serum antibody detection and a 78% concordance for platelet-associated antibody detection, with an 8-12% incidence of false positive or negative results.
在本研究中，我们比较了两种检测免疫性血小板减少性紫癜（ITP）中血小板结合型和血清中自身抗体GpIIb/IIIa的检测方法。发现两种检测方法具有相似的灵敏度，但血小板抗原单克隆抗体固定法（MAIPA）比免疫珠试验更具可重复性。MAIPA和免疫珠试验在血清抗体检测结果上的一致性为81%，在血小板相关抗体检测上的一致性为78%，假阳性或假阴性结果的发生率为8%-12%。
