刚地弓形虫重组抗原H4和H11：用于酶联免疫吸附测定法检测猪弓形虫病
Toxoplasma gondii recombinant antigens H4 and H11: use in ELISAs for detection of toxoplasmosis in swine.
作者信息
Andrews C D, Dubey J P, Tenter A M, Webert D W
机构信息
USDA, Food Safety and Inspection Service, Athens, GA 30605, USA.
出版信息
Vet Parasitol. 1997 Jun;70(1-3):1-11. doi: 10.1016/s0304-4017(96)01154-5.
Toxoplasma gondii recombinant antigens H4 and H11 were assessed for their potential for use in ELISA for diagnosis of toxoplasmosis in swine. The antigens were evaluated with sera from young pigs experimentally infected with T. gondii. Results were compared with ELISAs based on a native T. gondii antigen extract. Although recombinant antigen ELISAs showed a sharp rise in response with some sera very early after infection, they were relatively non-reactive with late (chronic) infection sera.
对弓形虫重组抗原H4和H11用于酶联免疫吸附测定（ELISA）诊断猪弓形虫病的潜力进行了评估。用实验性感染弓形虫的幼猪血清对这些抗原进行了评估。将结果与基于天然弓形虫抗原提取物的ELISA结果进行了比较。虽然重组抗原ELISA在感染后很早的时候对一些血清的反应有急剧上升，但它们对晚期（慢性）感染血清的反应相对较弱。
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