牛弓形虫感染血清学诊断用重组速殖子抗原蛋白 GRA7 的制备与评价。
Production and evaluation of recombinant granule antigen protein GRA7 for serodiagnosis of Toxoplasma gondii infection in cattle.
机构信息
1 Military Veterinary Institute , Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, China .
出版信息
Foodborne Pathog Dis. 2014 Sep;11(9):734-9. doi: 10.1089/fpd.2014.1749. Epub 2014 Jul 23.
The precise detection of Toxoplasma gondii infection in cattle has important public health significance. In the present study, recombinant granule antigen protein GRA7 was evaluated as a potential diagnostic marker for T. gondii infection in cattle by an indirect enzyme-linked immunosorbent assay (ELISA) using the reference serum samples determined by immunofluorescence assay and Western blotting, showing that GRA7-ELISA has a sensitivity of 96.4%, and a specificity of 98.6%. The detection results by GRA7- and Toxoplasma lysate antigen-based ELISAs were compared, indicating that no significant difference (p>0.05) and perfect agreement (κ=0.74) was observed between the two tests. Receiver operating characteristic analysis showed a relative sensitivity and specificity of 100% and 97.3% at the cut-off value of 0.3 for GRA7-ELISA. These data demonstrate that GRA7 is a promising serodiagnostic marker for T. gondii infection in cattle.
准确检测牛弓形体感染具有重要的公共卫生意义。在本研究中，通过间接酶联免疫吸附试验（ELISA），使用免疫荧光法和 Western blot 确定的参考血清样本评估重组颗粒抗原蛋白 GRA7 作为牛弓形体感染的潜在诊断标志物，结果表明 GRA7-ELISA 的敏感性为 96.4%，特异性为 98.6%。比较了基于 GRA7 和弓形虫裂解抗原的 ELISA 的检测结果，表明两种检测方法之间没有显著差异（p>0.05），具有很好的一致性（κ=0.74）。受试者工作特征分析显示，GRA7-ELISA 在截断值为 0.3 时的相对敏感性和特异性分别为 100%和 97.3%。这些数据表明 GRA7 是一种很有前途的牛弓形体感染血清学诊断标志物。
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